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Abstract

γ-butyrolactone and related signalling systems are found in and other actinobacteria where they control the production of secondary or specialized metabolites such as antibiotics. Genetic manipulation of these regulatory systems therefore leads to changes in the secondary metabolite profile of a strain and has been used to activate previously silent secondary metabolite gene clusters. However, there is no easy way to assess the presence of γ-butyrolactone-like systems in strains without whole-genome sequencing. We have therefore developed and tested a PCR screen that is able to detect homologues of the commonly co-located butenolide synthase and γ-butyrolactone receptor genes. This PCR screen could be employed for the screening of strain libraries to detect signalling systems without the necessity for whole-genome sequencing.

Funding
This study was supported by the:
  • Biotechnology and Biological Sciences Research Council (Award BB/M022765/1, BB/M017982/1)
    • Principle Award Recipient: ChristopheCorre
  • Natural Environment Research Council (Award NE/N019857/1, NE/S008721/1)
    • Principle Award Recipient: ChiaraBorsetto
  • H2020 Marie Skłodowska-Curie Actions (Award 765147)
    • Principle Award Recipient: ElizabethM. Wellington
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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/content/journal/acmi/10.1099/acmi.0.000661.v3
2023-09-12
2024-05-04
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