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Abstract

In this study we report the presence of streptomycin resistance gene within clinical isolates of where streptomycin is not used to treat Gram-negative infections. In total, 135 isolates were obtained for the study. PCR based detection of was performed in the study isolates followed by assessment of horizontal transferability. Cloning of was done in laboratory strain DH5α. Pre-cloning and post-cloning susceptibility of the strain was done for assessment of acquired resistance. Among tested isolates, 89 were found to harbour and it was encoded within a IncI1 type plasmid. Cloning experiments revealed the gene showed unusual non-susceptibility towards amikacin and gentamicin. The study highlighted that which has a role in streptomycin resistance, may also have a role in reduced susceptibility towards gentamicin and amikacin within a clinical setting.

Funding
This study was supported by the:
  • Indian Council of Medical Research (Award AMR/Fellowship/19/2019-ECD-II/ID No. 2019-3677 dated 21.08.2019)
    • Principle Award Recipient: JayalaxmiWangkheimayum
  • Jawaharlal Nehru Memorial Fund (Award SU-1/068/2018-19/89 dated December 4, 2017)
    • Principle Award Recipient: JayalaxmiWangkheimayum
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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2022-10-27
2024-12-10
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