1887

Abstract

(formerly designated as ) was first described in 2002 in the USA. Members of the genus can be part of the normal flora of the genitourinary tract, but have been implicated in invasive (mostly gynaecological) infections.

To the best of our knowledge, here we present the first case of infection in Belgium, in a young woman presenting with fever leading to second trimester septic abortion.

Despite its pathogenicity, remains an underrated cause of infections due to inherent difficulties with conventional laboratory methods. By extracting the bacterial DNA directly from the blood culture broth and performing a 16S ribosomal RNA gene sequence analysis we succeeded in identifying as the most probable cause of the septic abortion in our patient.

  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. The Microbiology Society waived the open access fees for this article.
Loading

Article metrics loading...

/content/journal/acmi/10.1099/acmi.0.000290
2021-12-09
2024-05-10
Loading full text...

Full text loading...

/deliver/fulltext/acmi/3/12/acmi000290.html?itemId=/content/journal/acmi/10.1099/acmi.0.000290&mimeType=html&fmt=ahah

References

  1. Boennelycke M, Christensen JJ, Arpi M, Krause S. Leptotrichia amnionii found in septic abortion in Denmark. Scand J Infect Dis 2007; 39:382–383 [View Article] [PubMed]
    [Google Scholar]
  2. Shukla SK, Meier PR, Mitchell PD, Frank DN, Reed KD. Leptotrichia amnionii sp. nov., a novel bacterium isolated from the amniotic fluid of a woman after intrauterine fetal demise. J Clin Microbiol 2002; 40:3346–3349 [View Article] [PubMed]
    [Google Scholar]
  3. Han YW, Shen T, Chung P, Buhimschi IA, Buhimschi CS. Uncultivated bacteria as etiologic agents of intra-amniotic inflammation leading to preterm birth. J Clin Microbiol 2009; 47:38–47 [View Article] [PubMed]
    [Google Scholar]
  4. Harwich MD Jr, Serrano MG, Fettweis JM, Alves JM, Reimers MA et al. Genomic sequence analysis and characterization of Sneathia amnii sp. nov. BMC Genomics 2012; 13:S4 [View Article]
    [Google Scholar]
  5. Nelson DE, Van Der Pol B, Dong Q, Revanna KV, Fan B et al. Characteristic male urine microbiomes associate with asymptomatic sexually transmitted infection. PLOS ONE 2010; 5:e14116 [View Article] [PubMed]
    [Google Scholar]
  6. Nawrot R, Kamieniarz K, Malinowska M, Józefiak A, Kedzia W et al. The prevalence of Leptotrichia amnionii in cervical swabs of HPV positive and negative women. Eur J Gynaecol Oncol 2010; 31:425–428 [PubMed]
    [Google Scholar]
  7. Fredricks DN, Fiedler TL, Thomas KK, Oakley BB, Marrazzo JM. Targeted PCR for detection of vaginal bacteria associated with bacterial vaginosis. J Clin Microbiol 2007; 45:3270–3276 [View Article] [PubMed]
    [Google Scholar]
  8. Haggerty CL, Totten PA, Tang G, Astete SG, Ferris MJ et al. Identification of novel microbes associated with pelvic inflammatory disease and infertility. Sex Transm Infect 2016; 92:441–446 [View Article] [PubMed]
    [Google Scholar]
  9. Ling Z, Kong J, Liu F, Zhu H, Chen X et al. Molecular analysis of the diversity of vaginal microbiota associated with bacterial vaginosis. BMC Genomics 2010; 11:488 [View Article] [PubMed]
    [Google Scholar]
  10. De Martino SJ, Mahoudeau I, Brettes JP, Piemont Y, Monteil H et al. Peripartum bacteremias due to Leptotrichia amnionii and Sneathia sanguinegens, rare causes of fever during and after delivery. J Clin Microbiol 2004; 42:5940–5943 [View Article] [PubMed]
    [Google Scholar]
  11. Doyle RM, Harris K, Kamiza S, Harjunmaa U, Ashorn U et al. Bacterial communities found in placental tissues are associated with severe chorioamnionitis and adverse birth outcomes. PLOS ONE 2017; 12:e0180167 [View Article] [PubMed]
    [Google Scholar]
  12. Thilesen CM, Nicolaidis M, Lökebö JE, Falsen E, Jorde AT et al. Leptotrichia amnionii, an emerging pathogen of the female urogenital tract. J Clin Microbiol 2007; 45:2344–2347 [View Article] [PubMed]
    [Google Scholar]
  13. Gardella C, Riley DE, Hitti J, Agnew K, Krieger JN et al. Identification and sequencing of bacterial rdnas in culture-negative amniotic fluid from women in premature labor. Am J Perinatol 2004; 21:319–323 [View Article] [PubMed]
    [Google Scholar]
  14. DiGiulio DB, Romero R, Amogan HP, Kusanovic JP, Bik EM et al. Microbial prevalence, diversity and abundance in amniotic fluid during preterm labor: a molecular and culture-based investigation. PLoS ONE 2008; 3:e3056. [View Article] [PubMed]
    [Google Scholar]
  15. Vitorino P, Varo R, Castillo P, Hurtado JC, Fernandes F et al. Sneathia amnii and maternal chorioamnionitis and stillbirth, mozambique. Emerg Infect Dis 2019; 25:1614–1616 [View Article] [PubMed]
    [Google Scholar]
  16. Fettweis JM, Serrano MG, Brooks JP, Edwards DJ, Girerd PH et al. The vaginal microbiome and preterm birth. Nat Med 2019; 25:1012–1021 [View Article] [PubMed]
    [Google Scholar]
  17. Hebb JK, Cohen CR, Astete SG, Bukusi EA, Totten PA. Detection of novel organisms associated with Salpingitis, by use of 16S rDNA polymerase chain reaction. J Infect Dis 2004; 190:2109–2120 [View Article] [PubMed]
    [Google Scholar]
  18. Gundi VAKB, Desbriere R, La Scola B. Leptotrichia amnionii and the Female reproductive tract. Emerg Infect Dis 2004; 10:2056–2057 [View Article] [PubMed]
    [Google Scholar]
  19. Gentile GL, Rupert AS, Carrasco LI, Garcia EM, Kumar NG et al. Identification of a cytopathogenic toxiCytopathogenic Toxin from Sneathia amnii. J Bacteriol 2020; 202:e00162-20 [View Article] [PubMed]
    [Google Scholar]
  20. Domann E, Hong G, Imirzalioglu C, Turschner S, Kühle J et al. Culture-independent identification of pathogenic bacteria and polymicrobial infections in the genitourinary tract of renal transplant recipients. J Clin Microbiol 2003; 41:5500–5510 [View Article] [PubMed]
    [Google Scholar]
  21. Decroix V, Goudjil S, Kongolo G, Mammeri H. “Leptotrichia amnionii”, a newly reported cause of early onset neonatal meningitis. J Med Microbiol 2013; 62:785–788 [View Article] [PubMed]
    [Google Scholar]
  22. Rao P, Chan M. Sneathia amnii epidural abscess following hysteroscopy: a case report [Poster session]. ECCMID 2017, Vienna, Austria; 201725 https://www.escmid.org/escmid_publications/escmid_elibrary/material/?mid=40546
  23. Goto M, Hitomi S, Ishii T. Bacterial arthritis caused by Leptotrichia amnionii. J Clin Microbiol 2007; 45:2082–2083 [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/acmi/10.1099/acmi.0.000290
Loading
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error