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Volume 3, Issue 11

Veterinary clinic surfaces as reservoirs of multi-drug- and biocide-resistant Gram-negative bacteria Open Access

Abstract

This cross-sectional study was carried out to determine the common Gram-negative bacteria (GNB) contaminating veterinary clinic environments, and to evaluate the susceptibility of the isolates to commonly used antibiotics and biocides. A total of 62 swab samples were collected from different frequently touched surfaces in the 4 veterinary clinics visited. The samples were processed for isolation and identification of GNB using standard microbiological procedures. The susceptibility of the isolates to disinfectants and antibiotics was determined using agar dilution and disc diffusion techniques, respectively. A total of 114 GNB were isolated from the 4 clinics with isolation rates of 21.9, 22.8, 23.7 and 31.6% in clinics A, B, C and D, respectively. The surfaces of treatment tables were more contaminated (16.7 %) than receptionist/clinician desks (15.8%), weighing balances (10.5 %), door handles (7.9 %), drip stands (7.9 %), handwashing basins (7.0 %) and client chairs (7.0%). The surface-contaminating isolates were distributed into 20 genera, with members of predominating (=97). Fifty-nine per cent of the isolates were resistant to the disinfectant Septol, while 5.3 and 0.9% were resistant to Purit and Dettol disinfectants, respectively. Multiple drug resistance was observed among 99% of the isolates with approximately 100% resistance to beta-lactams. Phenotypic expression of extended-spectrum (3.5 %) and AmpC beta-lactamase (38.6 %) production was detected. These findings highlight the role of clinic environments in serving as reservoirs for potential pathogens and sources for the spread of multi-drug resistant GNB.

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Keyword(s): infection , biocides , Gram-negative bacteria , pathogens , surfaces and veterinary clinics
© 2021 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution NonCommercial License. The Microbiology Society waived the open access fees for this article.
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2021-11-02
2024-05-13
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References

  1. Nunez S, Moreno A, Green K, Villar J. The stethoscope in the emergency department: a vector of infection?. Epidemiol Infect 2000; 124:233–237 [View Article] [PubMed]
     [Google Scholar]
  2. Yv D, Bik EM, Hochstenbach-Vernooij S, GJvd V, Savelkoul PHM. Management of an outbreak of Enterobacter cloacae in a neonatal unit using simple preventative measures. J Hosp Infect 2002; 51:21–26 [View Article] [PubMed]
     [Google Scholar]
  3. Aksoy E, Boag A, Brodbelt D, Grierson J. Evaluation of surface contamination with staphylococci in a veterinary hospital using a quantitative microbiological method. J Small Anim Pract 2010; 51:574–580 [View Article] [PubMed]
     [Google Scholar]
  4. KuKanich KS, Ghosh A, Skarbek JV, Lothamer KM, Zurek L. Surveillance of bacterial contamination in small animal veterinary hospitals with special focus on antimicrobial resistance and virulence traits of enterococci. J Am Vet Med Assoc 2012; 240:437–445 [View Article] [PubMed]
     [Google Scholar]
  5. Weese JS, Goth K, Ethier M, Boehnke K. Isolation of methicillin-resistant Staphylococcus aureus from the environment in a veterinary teaching hospital. J Vet Int Med 2004; 18:468–470 [View Article]
     [Google Scholar]
  6. Aklilu E, Zakaria Z, Hassan L, Hui CC. Molecular relatedness of methicillin-resistant s. aureus isolates from staff, environment and pets at university veterinary hospital in malaysia. PLoS ONE 2012; 7:e43329 [View Article]
     [Google Scholar]
  7. Benedict KM, Morley PS, Van Metre DC. Characteristics of biosecurity and infection control programs at veterinary teaching hospitals. J Am Vet Med Assoc 2008; 233:767–773 [View Article] [PubMed]
     [Google Scholar]
  8. Magiorakos A-. P, Srinivasan A, Carey RB, Carmeli Y, Falagas ME. Monnet,Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: an international expert proposal for interim standard definitions for acquired resistance. Clin MicrobiolInfect 2012; 18:268–281 [View Article]
     [Google Scholar]
  9. Humayoun SB, Hiott LM, Gupta SK, Barrett JB. Woodley TAet al.An assay for determining the susceptibility of Salmonella isolates to commercial and household biocides. PLoS ONE 2018; 13:e0209072 [View Article] [PubMed]
     [Google Scholar]
  10. Tuerena I, Williams NJ, Nuttall T, Pinchbeck G. Antimicrobial-resistant Escherichia coli in hospitalised companion animals and their hospital environment. J Small Anim Pract 2016; 57:339–347 [View Article]
     [Google Scholar]
  11. El-Mahmood AM, Doughari JH. Bacteriological examination of some diluted disinfectants routinely used in the Specialist Hospital Yola, Nigeria. AfrJPharm Pharmacol 2009; 3:185–190
     [Google Scholar]
  12. SheridanÀ LM, Duffy G, Fanning S, Burgess C. The potential for biocide tolerance in Escherichia coli and its impact on the response to food processing stresses. Food Control 2012; 26:98–106 [View Article]
     [Google Scholar]
  13. Capita R, Riesco-Peláez F, Alonso-Hernando A, Alonso-Calleja C. Exposure to sub-lethal concentrations of food-grade biocides influences the ability to formbiofilm, the resistance to antimicrobials and the ultrastructure of Escherichia coliATCC 12806. Appl Environ Microbiol 201302283–02213
     [Google Scholar]
  14. Molina-González D, Alonso-Calleja C, Alonso-Hernando A, Capita R. Effect of sub-lethal concentrations of biocides on the susceptibility to antibiotics of multi-drug resistant Salmonella enteric strains. Food Control 2014; 40:329–334 [View Article]
     [Google Scholar]
  15. Gantzhorn MR, Pedersen K, Olsen JE, Thomsen LE. Biocide and antibiotic susceptibility of Salmonella isolates obtained before and after cleaning at six Danish pig slaughter houses. International Journal of Food Microbiology 2014; 181:53–59 [View Article]
     [Google Scholar]
  16. Tageo.com A Database of Geographic Coordinate Information; 2021 https://www.tageo.com/
  17. Parija SC. Textbook of Practical Microbiology, 1st edn Delhi, India: Ahuja Publishers; 2006
     [Google Scholar]
  18. Clinical and Laboratory Standards Institute Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically; Approved Standard. Tenth Edition. Clinical and Laboratory Standards Institute document M07-A10 Clinical and Laboratory Standards Institute, 950 West Valley Road, Suite 2500, Wayne, Pennsylvania 19087 USA: 2015
     [Google Scholar]
  19. Anyanwu MU. Ukagwu LA,Chah KF.Antibiotic resistance profile of aerobic bacteria from environmental surfaces of some veterinary clinics in Enugu State, Nigeria. J Vet Appl Sci 2015; 5:36–43
     [Google Scholar]
  20. Bhatta DR, Hamal D, Shrestha R, Subramanya SH. Baral Net al.Bacterial contamination of frequently touched objects in a tertiary care hospital of Pokhara, Nepal: how safe are our hands?. Antimicrob Resist Infect Control 2018; 7:97 [View Article] [PubMed]
     [Google Scholar]
  21. Maillard J-. Y. n.d Bacterial target sites for biocide action. J Appl Microbiol Symp 92:16S–27S
     [Google Scholar]
  22. Maillard J-. Y. Bacterial resistance to biocides in the healthcare environment: should it be of genuine concern?. J Hosp Infect 2007; 65:60–72 [View Article] [PubMed]
     [Google Scholar]
  23. SCENIHR Scientific Committee on Emerging and Newly Identified Health Risks: Effects of Biocides on antibiotic resistance; 2009 http://copublications.greenfacts.org/en/biocides-antibiotic-resistance/ and at: http://ec.europa.eu/health/opinions/en/biocides-antibiotic-resistance/
  24. Munita JM, Arias CA. Mechanisms of Antibiotic Resistance. Microbiol Spectr 2016; 4: [View Article]
     [Google Scholar]
  25. Moawad AA, Hotzel H, Neubauer H, Ehricht R, Monecke S. Antimicrobial resistance in Enterobacteriaceae from healthy broilers in Egypt: emergence of colistin-resistant and extended-spectrum β-lactamase-producing Escherichia coli. Gut Pathog 2018; 10:39 [View Article] [PubMed]
     [Google Scholar]
  26. Livermore DM, Canton R, Gniadkowski M, Nordmann P, Rossolini GM. CTX-M: changing the face of ESBLs in Europe. J Antimicrob Chemother 2007; 59:165–174 [View Article] [PubMed]
     [Google Scholar]
  27. Karisik E, Ellington MJ, Pike R, Warren RE, Livermore DM. Molecular characterization of plasmids encoding CTX-M-15 beta-lactamases from Escherichia coli strains in the United Kingdom. J Antimicrob Chemother 2006; 58:665–668 [View Article] [PubMed]
     [Google Scholar]
  28. Yehualaeshet T. Cross-sectional study: Use of Antimicrobials in theVeterinary Clinics and Antibiotic Resistance. Intern J Vet Anim Med 2019; 2:119
     [Google Scholar]
  29. Sserwadda I, Lukenge M, Mwambi B, Mboowa G, al WA. Microbial contaminants isolated from items and work surfaces in the post-operative ward at Kawolo general hospital, Uganda. BMC Infect Dis 2018; 18:68 [View Article] [PubMed]
     [Google Scholar]
  30. Glickman L. Veterinary nosocomial (hospital-acquired) Klebsiella infections. J Am Vet Med Assoc 1981; 179:1389–1392 [PubMed]
     [Google Scholar]
  31. Baumgartner A, Schifferli D, Spiess B. Epidemiological study of a multi-resistant Enterobacter cloacae causing iatrogenic infection in a surgical unit [in German]. Zentralbl Veterinarmed B 1984; 31:73–77 [PubMed]
     [Google Scholar]
  32. Sanchez S, McCrackin Stevenson MA, Hudson CR, Maier M, Buffington T. Characterization of multidrug-resistant Escherichia coli isolates associated with nosocomial infections in dogs. J Clin Microbiol 2002; 40:3586–3595 [View Article] [PubMed]
     [Google Scholar]
  33. Rycroft AN, Garside LH. Actinobacillus species and their role in animal disease. The Vet J 2000; 159:18–36 [View Article]
     [Google Scholar]
  34. Loy JD, Brodersen BW. Moraxella spp. isolated from field outbreaks of infectious bovine keratoconjunctivitis: a retrospective study of case submissions from 2010 to 2013. J Vet Diagn Invest 2014; 26:761–768 [View Article] [PubMed]
     [Google Scholar]
  35. Seid A. Review on infectious bovine keratoconjunctivitis and its economic impacts in cattle. Dairy and Vet Sci J 2019; 9:555774 [View Article]
     [Google Scholar]
  36. Kim KT, Lee SH, Kwak D. Identification of Moraxella lacunata from pulmonary abscesses in three zoo herbivores. J Vet Med Sci 2018; 80:1914–1917 [View Article]
     [Google Scholar]
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Veterinary clinic surfaces as reservoirs of multi-drug- and biocide-resistant Gram-negative bacteria
Access Microbiology 3, 000277 (2021); https://doi.org/10.1099/acmi.0.000277
/content/journal/acmi/10.1099/acmi.0.000277
/content/journal/acmi/10.1099/acmi.0.000277
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