Comparing conventional, biochemical and genotypic methods for accurate identification of Klebsiella pneumoniae in Sudan

Einas A. Osman; Nagwa El-Amin; Emad A. E. Adrees; Leena Al-Hassan; Maowia Mukhtar

doi:10.1099/acmi.0.000096

Volume 2, Issue 3

Short Communication

Open Access

Comparing conventional, biochemical and genotypic methods for accurate identification of Klebsiella pneumoniae in Sudan

Einas A. Osman¹, Nagwa El-Amin², Emad A. E. Adrees³, Leena Al-Hassan^4,† and Maowia Mukhtar^1,†
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Affiliations: ¹ Bioscience Research Institute, Ibn-Sina University, Aljerif West, Khartoum, Sudan ² Microbiology Department, College of Medicine, Al-Qassim University, Al-Mulida, Saudi Arabia ³ Microbiology Department, College of Medicine, Alribat University Hospital, Khartoum, Sudan ⁴ Department of Global Health and Infection, Brighton and Sussex Medical School, Brighton BN1 9PX, UK
*Correspondence: Leena Al-Hassan, [email protected]

† These authors contributed equally to this work
Published: 10 February 2020 https://doi.org/10.1099/acmi.0.000096

Abstract

Klebsiella pneumoniae is recognized as one of the most important healthcare-associated pathogens worldwide due to its tendency to develop antibiotic resistance and cause fatal outcomes. Bacterial identification methods such as culture and biochemical tests are routinely used with limited accuracy in many low- and middle-income countries, including Sudan. The aim of this study was to test the accuracy of identification of K. pneumoniae in Khartoum, Sudan. Two hundred and fifty K. pneumoniae isolates were collected and identified using conventional phenotypic methods, biochemically using API 20E and genotypically by amplification of 16S−23S rDNA and sequencing of rpoB, gapA and pgi. Only 139 (55.6 %) of the isolates were confirmed as K. pneumoniae genotypically by PCR and 44.4 % were identified as non- K. pneumoniae . The results demonstrate that the identification panels used by the hospitals were inaccurately identifying K. pneumonia and led to overestimation of the prevalence of this organism. The current identification methods used in Khartoum hospitals are highly inaccurate, and therefore we recommend the use of a comprehensive biochemical panel or molecular methods, when possible, for accurate identification of K. pneumoniae .

Received: 03/10/2019
Accepted: 28/11/2019
Published Online: 10/02/2020

Keyword(s): Identification methods , Klebsiella pneumoniae and Sudan

Funding

This study was supported by the:

Association of Physicians of Great Britain and Ireland
- Principle Award Recipient: Leena Al-Hassan

This is an open-access article distributed under the terms of the Creative Commons Attribution License.

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Comparing conventional, biochemical and genotypic methods for accurate identification of Klebsiella pneumoniae in Sudan

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Volume 2, Issue 3

Short Communication

Open Access

Comparing conventional, biochemical and genotypic methods for accurate identification of Klebsiella pneumoniae in Sudan

Abstract

Funding

Supplementary material 1

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