1887

Abstract

is recognized as one of the most important healthcare-associated pathogens worldwide due to its tendency to develop antibiotic resistance and cause fatal outcomes. Bacterial identification methods such as culture and biochemical tests are routinely used with limited accuracy in many low- and middle-income countries, including Sudan. The aim of this study was to test the accuracy of identification of in Khartoum, Sudan. Two hundred and fifty isolates were collected and identified using conventional phenotypic methods, biochemically using API 20E and genotypically by amplification of 16S−23S rDNA and sequencing of , and . Only 139 (55.6 %) of the isolates were confirmed as genotypically by PCR and 44.4 % were identified as non-. The results demonstrate that the identification panels used by the hospitals were inaccurately identifying and led to overestimation of the prevalence of this organism. The current identification methods used in Khartoum hospitals are highly inaccurate, and therefore we recommend the use of a comprehensive biochemical panel or molecular methods, when possible, for accurate identification of .

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2020-02-10
2020-02-28
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