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Volume 10, Issue 5

Discovery and genetic characterization of novel paramyxoviruses from small mammals in Hubei Province, Central China Open Access

Abstract

Paramyxoviruses are a group of single-stranded, negative-sense RNA viruses, some of which are responsible for acute human disease, including parainfluenza virus, measles virus, Nipah virus and Hendra virus. In recent years, a large number of novel paramyxoviruses, particularly members of the genus , have been discovered in wild mammals, suggesting that the diversity of paramyxoviruses may be underestimated. Here we used hemi-nested reverse transcription PCR to obtain 190 paramyxovirus sequences from 969 small mammals in Hubei Province, Central China. These newly identified paramyxoviruses were classified into four clades: genera , , and , with most of them belonging to the genus . Using Illumina sequencing and Sanger sequencing, we successfully recovered six near-full-length genomes with different genomic organizations, revealing the more complex genome content of paramyxoviruses. Co-divergence analysis of jeilongviruses and their known hosts indicates that host-switching occurred more frequently in the evolutionary histories of the genus . Together, our findings demonstrate the high prevalence of paramyxoviruses in small mammals, especially jeilongviruses, and highlight the diversity of paramyxoviruses and their genome content, as well as the evolution of jeilongviruses.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): co-divergence , diversity , genome organization , Jeilongvirus and Paramyxovirus
Funding
This study was supported by the:
  • the Translational Medicine and Interdisciplinary Research Joint Fund of Zhongnan Hospital of Wuhan University (Award ZNLH202203)
    • Principle Award Recipient: NotApplicable
  • the Translational Medicine and Interdisciplinary Research Joint Fund of Zhongnan Hospital of Wuhan University (Award ZNLH201905)
    • Principle Award Recipient: NotApplicable
  • the Young Elite Scientist Sponsorship Program by CAST (Award YESS20200394)
    • Principle Award Recipient: Liang-junChen
  • National Key Research and Development Program of China (Award 2018YFE0204500)
    • Principle Award Recipient: Yi-rongLi
  • National Natural Science Foundation of China (Award 82000021)
    • Principle Award Recipient: Liang-junChen
  • National Natural Science Foundation of China (Award 81991220308)
    • Principle Award Recipient: WeiHou
  • National Natural Science Foundation of China (Award U20A20396)
    • Principle Award Recipient: WeiHou
  • National Key R&D Program of China (Award 2023YFC2605504)
    • Principle Award Recipient: NotApplicable
© 2024 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2024-05-03
2024-05-18
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Discovery and genetic characterization of novel paramyxoviruses from small mammals in Hubei Province, Central China
M Gen 10, 001229 (2024); https://doi.org/10.1099/mgen.0.001229
/content/journal/mgen/10.1099/mgen.0.001229
/content/journal/mgen/10.1099/mgen.0.001229
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