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Volume 9, Issue 6

Transconjugant range of PromA plasmids in microbial communities is predicted by sequence similarity with the bacterial host chromosome Open Access

Abstract

Nucleotide sequence similarity, including -mer plasmid composition, has been used for prediction of plasmid evolutionary host range, representing the hosts in which a plasmid has replicated at some point during its evolutionary history. However, the relationships between the bacterial taxa of experimentally identified transconjugants and the predicted evolutionary host ranges are poorly understood. Here, four different PromA group plasmids showing different -mer compositions were used as model plasmids. Filter mating assays were performed with a donor harbouring plasmids and recipients of bacterial communities extracted from environmental samples. A broad range of transconjugants was obtained with different bacterial taxa. A calculation of the dissimilarities in -mer compositions as Mahalanobis distance between the plasmid and its sequenced transconjugant chromosomes revealed that each plasmid and transconjugant were significantly more similar than the plasmid and other non-transconjugant chromosomes. These results indicate that plasmids with different -mer compositions clearly have different host ranges to which the plasmid will be transferred and replicated. The similarity of the nucleotide compositions could be used for predicting not only the plasmid evolutionary host range but also future host ranges.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): conjugation , host range , nucleotide compositions , Plasmid and PromA
Funding
This study was supported by the:
  • Ohsumi Frontier Science Foundation
    • Principle Award Recipient: MasakiShintani
  • Toyota Physical and Chemical Research Institute
    • Principle Award Recipient: MasakiShintani
  • Asahi Glass Foundation
    • Principle Award Recipient: MasakiShintani
  • Japan Agency for Medical Research and Development (Award JP21wm0225008)
    • Principle Award Recipient: MasakiShintani
  • Japan Agency for Medical Research and Development (Award JP21wm0325022)
    • Principle Award Recipient: MasakiShintani
  • Japan Society for the Promotion of Science (Award Jp22J12723)
    • Principle Award Recipient: MahoTokuda
  • Japan Society for the Promotion of Science (Award JP23H02124)
    • Principle Award Recipient: MasakiShintani
  • Japan Society for the Promotion of Science (Award JP20KK0128)
    • Principle Award Recipient: MasakiShintani
  • Japan Society for the Promotion of Science (Award JP19H05686)
    • Principle Award Recipient: MasakiShintani
  • Japan Society for the Promotion of Science (Award JP19H02869)
    • Principle Award Recipient: MasakiShintani
  • Japan Society for the Promotion of Science (Award JP16H06279)
    • Principle Award Recipient: MasakiShintani
© 2023 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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2023-06-21
2024-05-09
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Transconjugant range of PromA plasmids in microbial communities is predicted by sequence similarity with the bacterial host chromosome
M Gen 9, 001043 (2023); https://doi.org/10.1099/mgen.0.001043
/content/journal/mgen/10.1099/mgen.0.001043
/content/journal/mgen/10.1099/mgen.0.001043
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