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Volume 9, Issue 3

Dissemination of carbapenemase-producing in Ireland from 2012 to 2017: a retrospective genomic surveillance study Open Access

Abstract

The spread of carbapenemase-producing (CPE) is of major public health concern. The transmission dynamics of CPE in hospitals, particularly at the national level, are not well understood. Here, we describe a retrospective nationwide genomic surveillance study of CPE in Ireland between 2012 and 2017. We sequenced 746 national surveillance CPE samples obtained between 2012 and 2017. After clustering the sequences, we used thresholds based on pairwise SNPs, and reported within–host diversity along with epidemiological data to infer recent putative transmissions. All clusters in circulating clones, derived from high-resolution phylogenies, of a species (, , , , and ) were individually examined for evidence of transmission. Antimicrobial resistance trends over time were also assessed. We identified 352 putative transmission events in six species including widespread and frequent transmissions in three species. We detected putative outbreaks in 4/6 species with three hospitals experiencing prolonged outbreaks. The gene was the main cause of carbapenem resistance in Ireland in almost all species. An expansion in the number of sequence types carrying was an additional cause of the increasing prevalence of carbapenemase-producing and .

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): carbapenemase-producing Enterobacterales (CPE) , hospital transmission , Ireland national survey , SNP-based phylogeny and whole genome analysis
Funding
This study was supported by the:
  • MRC Centre for Global Infectious Disease Analysis (Award MR/R015600/1)
    • Principle Award Recipient: JohnA. Lees
  • National Institute for Health Research (NIHR) Senior Investigator Award
    • Principle Award Recipient: SharonJ. Peacock
  • Wellcome Trust Sir Henry Postdoctoral Fellowship (Award 110243/Z/15/Z)
    • Principle Award Recipient: CatherineLudden
  • Health Innovation Challenge Fund (Award WT098600, HICF-T5- 391 342)
    • Principle Award Recipient: CatherineLudden
© 2023 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2024-05-04
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Dissemination of carbapenemase-producing Enterobacterales in Ireland from 2012 to 2017: a retrospective genomic surveillance study
M Gen 9, 000924 (2023); https://doi.org/10.1099/mgen.0.000924
/content/journal/mgen/10.1099/mgen.0.000924
/content/journal/mgen/10.1099/mgen.0.000924
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