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Volume 7, Issue 12

Genome reorganization during emergence of host-associated Open Access

Abstract

is a rapid growing, free-living species of bacterium that also causes lung infections in humans. Human infections are usually acquired from the environment; however, dominant circulating clones (DCCs) have emerged recently in both subsp. and subsp. that appear to be transmitted among humans and are now globally distributed. These recently emerged clones are potentially informative about the ecological and evolutionary mechanisms of pathogen emergence and host adaptation. The geographical distribution of DCCs has been reported, but the genomic processes underlying their transition from environmental bacterium to human pathogen are not well characterized. To address this knowledge gap, we delineated the structure of subspecies and using genomic data from 200 clinical isolates of from seven geographical regions. We identified differences in overall patterns of lateral gene transfer (LGT) and barriers to LGT between subspecies and between environmental and host-adapted bacteria. We further characterized genome reorganization that accompanied bacterial host adaptation, inferring selection pressures acting at both genic and intergenic loci. We found that both subspecies encode an expansive pangenome with many genes at rare frequencies. Recombination appears more frequent in subsp. than in subsp. , consistent with prior reports. We found evidence suggesting that phage are exchanged between subspecies, despite genetic barriers evident elsewhere throughout the genome. Patterns of LGT differed according to niche, with less LGT observed among host-adapted DCCs versus environmental bacteria. We also found evidence suggesting that DCCs are under distinct selection pressures at both genic and intergenic sites. Our results indicate that host adaptation of was accompanied by major changes in genome evolution, including shifts in the apparent frequency of LGT and impacts of selection. Differences were evident among the DCCs as well, which varied in the degree of gene content remodelling, suggesting they were placed differently along the evolutionary trajectory toward host adaptation. These results provide insight into the evolutionary forces that reshape bacterial genomes as they emerge into the pathogenic niche.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): evolution , lateral gene transfer , Mycobacterium abscessus , NTM infection and recombination
Funding
This study was supported by the:
  • National Science Foundation (Award DGE-1747503)
    • Principle Award Recipient: MadisonYoungblom
  • National Institutes of Health (Award T32AI55391)
    • Principle Award Recipient: LindseyL Bohr
  • National Institutes of Health (Award R01AI113287)
    • Principle Award Recipient: LindseyL Bohr
  • National Institutes of Health (Award R01AI113287)
    • Principle Award Recipient: CaitlinS Pepperell
© 2021 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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References

  1. Lopeman RC, Harrison J, Desai M, Cox JAG. Mycobacterium abscessus: environmental bacterium turned clinical nightmare. Microorganisms 2019; 7:90 [View Article]
     [Google Scholar]
  2. Johansen MD, Herrmann J-L, Kremer L. Non-tuberculous mycobacteria and the rise of Mycobacterium abscessus. Nat Rev Microbiol 2020; 18:392–407 [View Article]
     [Google Scholar]
  3. Moore M, Frerichs JB. An unusual acid-fast infection of the knee with subcutaneous, abscess-like lesions of the gluteal region; report of a case with a study of the organism, Mycobacterium abscessus, n. sp. J Invest Dermatol 1953; 20:133–169 [View Article] [PubMed]
     [Google Scholar]
  4. Floto RA, Olivier KN, Saiman L, Daley CL, Herrmann J-L et al. US cystic fibrosis foundation and European cystic fibrosis society consensus recommendations for the management of non-tuberculous mycobacteria in individuals with cystic fibrosis: executive summary. Thorax 2016; 71:88–90 [View Article] [PubMed]
     [Google Scholar]
  5. Griffith DE, Brown-Elliott BA, Benwill JL, Wallace RJ. Mycobacterium abscessus. “Pleased to meet you, hope you guess my name…”. Ann Am Thorac Soc 2015; 12:436–439 [View Article] [PubMed]
     [Google Scholar]
  6. Jarand J, Levin A, Zhang L, Huitt G, Mitchell JD et al. Clinical and microbiologic outcomes in patients receiving treatment for Mycobacterium abscessus pulmonary disease. Clin Infect Dis 2011; 52:565–571 [View Article] [PubMed]
     [Google Scholar]
  7. Choi H, Jhun BW, Kim S-Y, Kim DH, Lee H et al. Treatment outcomes of macrolide-susceptible Mycobacterium abscessus lung disease. Diagn Microbiol Infect Dis 2018; 90:293–295 [View Article] [PubMed]
     [Google Scholar]
  8. Bryant JM, Grogono DM, Rodriguez-Rincon D, Everall I, Brown KP et al. Emergence and spread of a human-transmissible multidrug-resistant nontuberculous mycobacterium. Science 2016; 354:751–757 [View Article] [PubMed]
     [Google Scholar]
  9. Bryant JM, Grogono DM, Greaves D, Foweraker J, Roddick I et al. Whole-genome sequencing to identify transmission of Mycobacterium abscessus between patients with cystic fibrosis: a retrospective cohort study. Lancet 2013; 381:1551–1560 [View Article] [PubMed]
     [Google Scholar]
  10. Davidson RM, Hasan NA, Epperson LE, Benoit JB, Kammlade SM et al. Population genomics of Mycobacterium abscessus from United States cystic fibrosis care centers. Ann Am Thorac Soc 2021 [View Article]
     [Google Scholar]
  11. Doyle RM, Rubio M, Dixon G, Hartley J, Klein N et al. Cross-transmission is not the source of new Mycobacterium abscessus infections in a multicenter cohort of cystic fibrosis patients. Clin Infect Dis 2020; 70:1855–1864 [View Article] [PubMed]
     [Google Scholar]
  12. Tortoli E, Kohl TA, Brown-Elliott BA, Trovato A, Leão SC et al. Emended description of Mycobacterium abscessus, Mycobacterium abscessus subsp. abscessus and Mycobacterium abscessus subsp. bolletii and designation of Mycobacterium abscessus subsp. massiliense comb. nov. Int J Syst Evol Microbiol 2016; 66:4471–4479 [View Article] [PubMed]
     [Google Scholar]
  13. Tan JL, Ng KP, Ong CS, Ngeow YF. Genomic comparisons reveal microevolutionary differences in Mycobacterium abscessus subspecies. Front Microbiol 2017; 8:2042 [View Article] [PubMed]
     [Google Scholar]
  14. Ripoll F, Pasek S, Schenowitz C, Dossat C, Barbe V et al. Non mycobacterial virulence genes in the genome of the emerging pathogen Mycobacterium abscessus. PLoS One 2009; 4:e5660 [View Article]
     [Google Scholar]
  15. Leão SC, Matsumoto CK, Carneiro A, Ramos RT, Nogueira CL et al. The detection and sequencing of a broad-host-range conjugative IncP-1β plasmid in an epidemic strain of Mycobacterium abscessus subsp. bolletii. PLoS One 2013; 8:e60746 [View Article]
     [Google Scholar]
  16. Dedrick RM, Aull HG, Jacobs-Sera D, Garlena RA, Russell DA et al. The prophage and plasmid mobilome as a likely driver of Mycobacterium abscessus diversity. mBio 2021; 12:e03441-20 [PubMed]
     [Google Scholar]
  17. Gutierrez MC, Brisse S, Brosch R, Fabre M, Omaïs B et al. Ancient origin and gene mosaicism of the progenitor of Mycobacterium tuberculosis. PLoS Pathog 2005; 1:e5 [View Article]
     [Google Scholar]
  18. Becq J, Gutierrez MC, Rosas-Magallanes V, Rauzier J, Gicquel B et al. Contribution of horizontally acquired genomic islands to the evolution of the tubercle bacilli. Mol Biol Evol 2007; 24:1861–1871 [View Article] [PubMed]
     [Google Scholar]
  19. Supply P, Marceau M, Mangenot S, Roche D, Rouanet C et al. Genomic analysis of smooth tubercle bacilli provides insights into ancestry and pathoadaptation of Mycobacterium tuberculosis. Nat Genet 2013; 45:172–179 [View Article] [PubMed]
     [Google Scholar]
  20. Sreevatsan S, Pan X, Stockbauer KE, Connell ND, Kreiswirth BN et al. Restricted structural gene polymorphism in the Mycobacterium tuberculosis complex indicates evolutionarily recent global dissemination. Proc Natl Acad Sci USA 1997; 94:9869–9874 [View Article] [PubMed]
     [Google Scholar]
  21. Hirsh AE, Tsolaki AG, DeRiemer K, Feldman MW, Small PM. Stable association between strains of Mycobacterium tuberculosis and their human host populations. Proc Natl Acad Sci USA 2004; 101:4871–4876 [View Article] [PubMed]
     [Google Scholar]
  22. Pepperell C, Hoeppner VH, Lipatov M, Wobeser W, Schoolnik GK et al. Bacterial genetic signatures of human social phenomena among M. tuberculosis from an aboriginal Canadian population. Mol Biol Evol 2010; 27:427–440 [View Article] [PubMed]
     [Google Scholar]
  23. Pepperell CS, Casto AM, Kitchen A, Granka JM, Cornejo OE et al. The role of selection in shaping diversity of natural M. tuberculosis populations. PLoS Pathog 2013; 9:e1003543 [View Article]
     [Google Scholar]
  24. Murray GGR, Charlesworth J, Miller EL, Casey MJ, Lloyd CT et al. Genome reduction is associated with bacterial pathogenicity across different scales of temporal and ecological divergence. Mol Biol Evol 2021; 38:1570–1579
     [Google Scholar]
  25. Weinert LA, Welch JJ. Why might bacterial pathogens have small genomes?. Trends Ecol Evol 2017; 32:936–947 [View Article]
     [Google Scholar]
  26. Stinear TP. Inights from the complete genome sequence of Mycobacterium marinum on the evolution of Mycobacterium tuberculosis. Genome Res 2008; 18:729–741 [PubMed]
     [Google Scholar]
  27. Ochman H. Genes lost and genes found: evolution of bacterial pathogenesis and symbiosis. Science 2001; 292:1096–1099 [View Article] [PubMed]
     [Google Scholar]
  28. Andrews S. FastQC: a Quality Control Tool for High Throughput Sequence Data 2010 http://www.bioinformatics.babraham.ac.uk/projects/fastqc/
  29. Krueger F. TrimGalore: a Wrapper Tool Around Cutadapt and FastQC to Consistently Apply Quality and Adapter Trimming to FastQ files 2015 https://www.bioinformatics.babraham.ac.uk/projects/trim_galore/
  30. Li H, Handsaker B, Wysoker A, Fennell T, Ruan J et al. The sequence alignment/map format and SAMtools. Bioinformatics 2009; 25:2078–2079 [View Article] [PubMed]
     [Google Scholar]
  31. Walker BJ, Abeel T, Shea T, Priest M, Abouelliel A et al. Pilon: an integrated tool for comprehensive microbial variant detection and genome assembly improvement. PLoS One 2014; 9:e112963 [View Article]
     [Google Scholar]
  32. Price MN. FastTree 2 – approximately maximum-likelihood trees for large alignments. PLoS One 2010; 5:e9490
     [Google Scholar]
  33. Harris SR. tree_gubbins: Clustering of Isolates on a Phylogenetic Tree Using a Spatial Scan Statistic 2017 https://github.com/simonrharris/tree_gubbins
  34. Menardo F, Loiseau C, Brites D, Coscolla M, Gygli SM et al. Treemmer: a tool to reduce large phylogenetic datasets with minimal loss of diversity. BMC Bioinformatics 2018; 19:164 [View Article] [PubMed]
     [Google Scholar]
  35. Eldholm V, Norheim G, von der Lippe B, Kinander W, Dahle UR et al. Evolution of extensively drug-resistant Mycobacterium tuberculosis from a susceptible ancestor in a single patient. Genome Biol 2014; 15:490 [View Article]
     [Google Scholar]
  36. Bankevich A, Nurk S, Antipov D, Gurevich AA, Dvorkin M et al. SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol 2012; 19:455–477 [View Article] [PubMed]
     [Google Scholar]
  37. Seemann T. Prokka: rapid prokaryotic genome annotation. Bioinformatics 2014; 30:2068–2069 [View Article]
     [Google Scholar]
  38. Gurevich A, Saveliev V, Vyahhi N, Tesler G. QUAST: quality assessment tool for genome assemblies. Bioinformatics 2013; 29:1072–1075 [View Article] [PubMed]
     [Google Scholar]
  39. Page AJ, Cummins CA, Hunt M, Wong VK, Reuter S et al. Roary: rapid large-scale prokaryote pan genome analysis. Bioinformatics 2015; 31:3691–3693 [View Article] [PubMed]
     [Google Scholar]
  40. Löytynoja A. Phylogeny-aware alignment with PRANK. Methods Mol Biol 2014; 1079:155–170
     [Google Scholar]
  41. Stamatakis A. RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 2014; 30:1312–1313 [View Article]
     [Google Scholar]
  42. Yu G, Smith DK, Zhu H, Guan Y, Lam TT et al. ggtree: an R package for visualization and annotation of phylogenetic trees with their covariates and other associated data. Methods Ecol Evol 2016; 8:28–36 [View Article]
     [Google Scholar]
  43. Croucher NJ, Page AJ, Connor TR, Delaney AJ, Keane JA et al. Rapid phylogenetic analysis of large samples of recombinant bacterial whole genome sequences using Gubbins. Nucleic Acids Res 2015; 43:e15 DOI: DOI: 10.1093/nar/gku1196 [PubMed]
     [Google Scholar]
  44. Hadfield J, Croucher NJ, Goater RJ, Abudahab K, Aanensen DM et al. Phandango: an interactive viewer for bacterial population genomics. Bioinformatics 2018; 34:292–293 [View Article] [PubMed]
     [Google Scholar]
  45. Kruskal WH, Wallis WA. Use of ranks in one-criterion variance analysis. J Am Stat Assoc 1952; 47:583–621 [View Article]
     [Google Scholar]
  46. Mann HB, Whitney DR. On a test of whether one of two random variables is stochastically larger than the other. Ann Math Statist 1947; 18:50–60 [View Article]
     [Google Scholar]
  47. Bonferroni CE. Il calcolo delle assicurazioni su gruppi di teste. Studi Onore Profr Salvatore Ortu Carboni 193513–60
     [Google Scholar]
  48. Mortimer TD, Pepperell CS. Genomic signatures of distributive conjugal transfer among mycobacteria. Genome Biol Evol 2014; 6:2489–2500 [View Article] [PubMed]
     [Google Scholar]
  49. Boritsch EC, Khanna V, Pawlik A, Honoré N, Navas VH et al. Key experimental evidence of chromosomal DNA transfer among selected tuberculosis-causing mycobacteria. Proc Natl Acad Sci USA 2016; 113:9876–9881 DOI: DOI: 10.1073/pnas.1604921113
    [Google Scholar]
  50. Parsons LM, Jankowski CS, Derbyshire KM. Conjugal transfer of chromosomal DNA in Mycobacterium smegmatis. Mol Microbiol 1998; 28:571–582 [View Article] [PubMed]
     [Google Scholar]
  51. Wang J, Derbyshire KM. Plasmid DNA transfer in Mycobacterium smegmatis involves novel DNA rearrangements in the recipient, which can be exploited for molecular genetic studies. Mol Microbiol 2004; 53:1233–1241 [View Article] [PubMed]
     [Google Scholar]
  52. Huson DH, Bryant D. Application of phylogenetic networks in evolutionary studies. Mol Biol Evol 2006; 23:254–267 [View Article] [PubMed]
     [Google Scholar]
  53. Bruen TC, Philippe H, Bryant D. A simple and robust statistical test for detecting the presence of recombination. Genetics 2006; 172:2665–2681 [View Article] [PubMed]
     [Google Scholar]
  54. Lawson DJ, Hellenthal G, Myers S, Falush D. Inference of population structure using dense haplotype data. PLoS Genet 2012; 8:e1002453 [View Article]
     [Google Scholar]
  55. Page AJ, Taylor B, Delaney AJ, Soares J, Seemann T et al. SNP-sites: rapid efficient extraction of SNPs from multi-FASTA alignments. Microb Genom 2016; 2:e000056 [View Article] [PubMed]
     [Google Scholar]
  56. Danecek P, Auton A, Abecasis G, Albers CA, Banks E et al. The variant call format and VCFtools. Bioinformatics 2011; 27:2156–2158 [View Article] [PubMed]
     [Google Scholar]
  57. De Mita S, Siol M. EggLib: processing, analysis and simulation tools for population genetics and genomics. BMC Genet 2012; 13:27 [View Article] [PubMed]
     [Google Scholar]
  58. Reis-Cunha JL, Bartholomeu DC, Manson AL, Earl AM, Cerqueira GC et al. ProphET, prophage estimation tool: a stand-alone prophage sequence prediction tool with self-updating reference database. PLoS One 2019; 14:e0223364 [View Article]
     [Google Scholar]
  59. Ondov BD, Treangen TJ, Melsted P, Mallonee AB, Bergman NH et al. Mash: fast genome and metagenome distance estimation using MinHash. Genome Biol 2016; 17:132 [View Article] [PubMed]
     [Google Scholar]
  60. Thorpe HA, Bayliss SC, Sheppard SK, Feil EJ. Piggy: a rapid, large-scale pan-genome analysis tool for intergenic regions in bacteria. Gigascience 2018; 7:1–11 [View Article] [PubMed]
     [Google Scholar]
  61. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. J Mol Biol 1990; 215:403–410 [View Article] [PubMed]
     [Google Scholar]
  62. Belda E, Moya A, Bentley S, Silva FJ. Mobile genetic element proliferation and gene inactivation impact over the genome structure and metabolic capabilities of Sodalis glossinidius, the secondary endosymbiont of tsetse flies. BMC Genomics 2010; 11:449 [View Article] [PubMed]
     [Google Scholar]
  63. Thorpe HA, Bayliss SC, Hurst LD, Feil EJ. Comparative analyses of selection operating on nontranslated intergenic regions of diverse bacterial species. Genetics 2017; 206:363–376
     [Google Scholar]
  64. Yang Z, Nielsen R. Estimating synonymous and nonsynonymous substitution rates under realistic evolutionary models. Mol Biol Evol 2000; 17:32–43 [View Article] [PubMed]
     [Google Scholar]
  65. Yang Z. PAML: a program package for phylogenetic analysis by maximum likelihood. Comput Appl Biosci 1997; 13:555–556 [View Article] [PubMed]
     [Google Scholar]
  66. Falush D, Stephens M, Pritchard JK. Inference of population structure using multilocus genotype data: dominant markers and null alleles. Mol Ecol Notes 2007; 7:574–578 [View Article] [PubMed]
     [Google Scholar]
  67. Sapriel G, Konjek J, Orgeur M, Bouri L, Frézal L et al. Genome-wide mosaicism within Mycobacterium abscessus: evolutionary and epidemiological implications. BMC Genomics 2016; 17:118 [View Article] [PubMed]
     [Google Scholar]
  68. Gray TA, Krywy JA, Harold J, Palumbo MJ, Derbyshire KM et al. Distributive conjugal transfer in mycobacteria generates progeny with meiotic-like genome-wide mosaicism, allowing mapping of a mating identity locus. PLoS Biol 2013; 11:e1001602 [View Article]
     [Google Scholar]
  69. Gray TA, Derbyshire KM. Blending genomes: distributive conjugal transfer in mycobacteria, a sexier form of HGT. Mol Microbiol 2018; 108:601–613 [View Article] [PubMed]
     [Google Scholar]
  70. Rocha EPC, Smith JM, Hurst LD, Holden MTG, Cooper JE et al. Comparisons of dN/dS are time dependent for closely related bacterial genomes. J Theor Biol 2006; 239:226–235 [View Article] [PubMed]
     [Google Scholar]
  71. Bar-On O, Mussaffi H, Mei-Zahav M, Prais D, Steuer G et al. Increasing nontuberculous mycobacteria infection in cystic fibrosis. J Cyst Fibros 2015; 14:53–62 [View Article] [PubMed]
     [Google Scholar]
  72. Roux A-L, Catherinot E, Ripoll F, Soismier N, Macheras E et al. Multicenter study of prevalence of nontuberculous mycobacteria in patients with cystic fibrosis in France. J Clin Microbiol 2009; 47:4124–4128 [View Article] [PubMed]
     [Google Scholar]
  73. Olivier KN, Weber DJ, Wallace RJ, Faiz AR, Lee J-H et al. Nontuberculous mycobacteria. Am J Respir Crit Care Med 2003; 167:828–834 [View Article]
     [Google Scholar]
  74. Choo SW, Wee WY, Ngeow YF, Mitchell W, Tan JL et al. Genomic reconnaissance of clinical isolates of emerging human pathogen Mycobacterium abscessus reveals high evolutionary potential. Sci Rep 2014; 4:4061 [View Article] [PubMed]
     [Google Scholar]
  75. Arredondo-Alonso S, Willems RJ, van Schaik W, Schürch AC. On the (im)possibility of reconstructing plasmids from whole-genome short-read sequencing data. Microb Genom 2017; 3:e000128 [View Article] [PubMed]
     [Google Scholar]
  76. Coros A, Callahan B, Battaglioli E, Derbyshire KM. The specialized secretory apparatus ESX-1 is essential for DNA transfer in Mycobacterium smegmatis. Mol Microbiol 2008; 69:794–808 [View Article] [PubMed]
     [Google Scholar]
  77. Flint JL, Kowalski JC, Karnati PK, Derbyshire KM. The RD1 virulence locus of Mycobacterium tuberculosis regulates DNA transfer in Mycobacterium smegmatis. Proc Natl Acad Sci USA 2004; 101:12598–12603 [View Article] [PubMed]
     [Google Scholar]
  78. Gray TA, Clark RR, Boucher N, Lapierre P, Smith C et al. Intercellular communication and conjugation are mediated by ESX secretion systems in mycobacteria. Science 2016; 354:347–350 [View Article] [PubMed]
     [Google Scholar]
  79. Mortimer TD, Weber AM, Pepperell CS. Evolutionary thrift: mycobacteria repurpose plasmid diversity during adaptation of type VII secretion systems. Genome Biol Evol 2017; 9:398–413 [View Article]
     [Google Scholar]
  80. Bohr LL, Mortimer TD, Pepperell CS. Lateral gene transfer shapes diversity of Gardnerella spp. Front Cell Infect Microbiol 2020; 10:293 [View Article] [PubMed]
     [Google Scholar]
  81. Georgiades K, Raoult D, El-Sayed N. Genomes of the most dangerous epidemic bacteria have a virulence repertoire characterized by fewer genes but more toxin-antitoxin modules. PLoS One 2011; 6:e17962 [View Article]
     [Google Scholar]
  82. Bryant JM, Brown KP, Burbaud S, Everall I, Belardinelli JM et al. Stepwise pathogenic evolution of Mycobacterium abscessus. Science 2021; 372:eabb8699 [View Article]
     [Google Scholar]
  83. Koeck J-L, Fabre M, Simon F, Daffé M, Garnotel E et al. Clinical characteristics of the smooth tubercle bacilli ‘Mycobacterium canettii’ infection suggest the existence of an environmental reservoir. Clin Microbiol Infect 2011; 17:1013–1019 [View Article]
     [Google Scholar]
  84. Murray GGR, Charlesworth J, Miller EL, Casey MJ, Lloyd CT et al. Genome reduction is associated with bacterial pathogenicity across different scales of temporal and ecological divergence. Mol Biol Evol 2021; 38:1570–1579
     [Google Scholar]
  85. Molina N, van Nimwegen E. Universal patterns of purifying selection at noncoding positions in bacteria. Genome Res 2008; 18:148–160 [View Article] [PubMed]
     [Google Scholar]
  86. Luo H, Tang J, Friedman R, Hughes AL. Ongoing purifying selection on intergenic spacers in group A streptococcus. Infect Genet Evol 2011; 11:343–348 [View Article] [PubMed]
     [Google Scholar]
  87. Degnan PH, Ochman H, Moran NA, Casadesús J. Sequence conservation and functional constraint on intergenic spacers in reduced genomes of the obligate symbiont Buchnera. PLoS Genet 2011; 7:e1002252 [View Article]
     [Google Scholar]
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Genome reorganization during emergence of host-associated Mycobacterium abscessus
M Gen 7, 000706 (2021); https://doi.org/10.1099/mgen.0.000706
/content/journal/mgen/10.1099/mgen.0.000706
/content/journal/mgen/10.1099/mgen.0.000706
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