1887

Abstract

Introduction:

is rarely associated with human diseases. Infection is usuallcaused by ingestion of contaminated food, and infant botulism is the most common clinical presentation.

Case Report:

Here we report a case of pneumonia by a non-toxigenic strain of in an Alzheimer 70-year-old male with sepsis in Rio de Janeiro, Brazil. The micro-organism was identified by phenotypical tests, mass spectrometry (MALDI-TOF), DNA amplification (PCR) and sequencing of the 16S rRNA gene. Testing for the presence of botulinum F toxin was made using multiplex PCR. Bioassay for a large number of colonies was performed in mice to evaluate the production of any lethal toxin, but the results were negative.

Conclusion:

To our knowledge, there are no cases of infection reported in Brazil and we highlight the importance of anaerobic lab tests in the standard routine of diagnosis.

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2016-07-11
2024-12-03
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References

  1. Bischoff A. 2012; [Clostridium infections. Lethal toxins: what chances are there?]. MMW Fortschr Med 154:26 (In German) [PubMed]
    [Google Scholar]
  2. CLSI 2014; Performance Standards for Antimicrobial Susceptibility Testing. Twenty-Fourth Informational Supplement. CLSI Document M100-S24 34:1–214
    [Google Scholar]
  3. De Medici D., Anniballi F., Wyatt G. M., Lindström M., Messelhäusser U., Aldus C. F., Delibato E., Korkeala H., Peck M. W. et al. 2009; Multiplex PCR for detection of botulinum neurotoxin-producing clostridia in clinical, food, and environmental samples. Appl Environ Microbiol 75:6457–6461 [View Article][PubMed]
    [Google Scholar]
  4. Fox J. G., Ge Z., Whary M. T., Erdman S. E., Horwitz B. H. 2011; Helicobacter hepaticus infection in mice: models for understanding lower bowel inflammation and cancer. Mucosal Immunol 4:22–30 [View Article][PubMed]
    [Google Scholar]
  5. Hall J. D., McCroskey L. M., Pincomb B. J., Hatheway C. L. 1985; Isolation of an organism resembling Clostridium barati which produces type F botulinal toxin from an infant with botulism. J Clin Microbiol 21:654–655[PubMed]
    [Google Scholar]
  6. Hannett G. E., Schaffzin J. K., Davis S. W., Fage M. P., Schoonmaker-Bopp D., Dumas N. B., Musser K. A., Egan C. 2014; Two cases of adult botulism caused by botulinum neurotoxin producing Clostridium baratii . Anaerobe 30C:178–180 [View Article][PubMed]
    [Google Scholar]
  7. Huang W. C., Lee W. S., Chang T., Ou T. Y., Lam C. 2012; Emphysematous cholecystitis complicating liver abscess due to Clostridium baratii infection. J Microbiol Immunol Infect 45:390–392 [View Article][PubMed]
    [Google Scholar]
  8. Iaria C., Stassi G., Salpietro D. C., La Mazza A., Silipigni L., Arena A., Costa G. B., Cascio A. 2007; Clostridium baratii bacteremia associated with Kawasaki syndrome. First case report. New Microbiol 30:481–484[PubMed]
    [Google Scholar]
  9. Krieg N. R., Ludwig W., Whitman W. B., Hedlund B. P., Paster B. J., Staley J. T., Ward N., Brown D. 2010 Bergey’s Manual of Systematic Bacteriology, 2nd edn. 4 New York, NY: Springer-Verlag; [CrossRef]
    [Google Scholar]
  10. Ngo J. T., Parkins M. D., Gregson D. B., Pitout J. D., Ross T., Church D. L., Laupland K. B. 2013; Population-based assessment of the incidence, risk factors, and outcomes of anaerobic bloodstream infections. Infection 41:41–48 [View Article][PubMed]
    [Google Scholar]
  11. Park Y., Choi J. Y., Yong D., Lee K., Kim J. M. 2009; Clinical features and prognostic factors of anaerobic infections: a 7-year retrospective study. Korean J Intern Med 24:13–18 [View Article][PubMed]
    [Google Scholar]
  12. Sebald M., Petit J. C. 1997 Laboratory Methods Anaerobic Bacteria and Their Identification, 2nd edn. pp 189–197 Paris: Institute Pasteur;
    [Google Scholar]
  13. Shu C. C., Yao M., Hung C. C., Ku S. C., Yu C. J., Chang Y. L. 2008; Lung abscess due to Clostridium baratii infection in a patient with invasive pulmonary aspergillosis. J Clin Microbiol 46:1153–1154 [View Article][PubMed]
    [Google Scholar]
  14. Somier J. 2002 Wadsworth Anaerobes Bacteriology Manual, 6 edn. Edited by Josiemies-Somier H. R., Summanen P., Citron D. M., Baron E. J., Wexler H. M., Finegold F. M. Belmont, CA, USA: Star Publishing Company;
    [Google Scholar]
  15. Tréhard H., Poujol I., Mazuet C., Blanc Q., Gillet Y., Rossignol F., Popoff M. R., Jourdan Da Silva N. 2016; A cluster of three cases of botulism due to Clostridium baratii type F, France, August 2015. Euro Surveill 21:4 [CrossRef]
    [Google Scholar]
  16. Tsybuliak G. N., Epifanov M. V. 2009; [Clostridial forms of anaerobic infections of wounds]. Vestn Khir Im I I Grek 168:111–115 (In Russian) [PubMed]
    [Google Scholar]
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