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Abstract

Introduction:

Intra-uterine infection is a major cause of spontaneous pre-term birth before 30 weeks of gestation. In addition, intra-amniotic inflammation is present in approximately 80 % of patients with acute cervical insufficiency. The microbiome is known to cause intra-amniotic infection causing pre-term birth. Recently, surveys of the microbial communities in the vagina or in amniotic fluid using sequencing of the 16s rRNA gene have been carried out. Here, we reported the microbiome compositions in mid-trimester amniotic fluids in two pregnant women with cervical insufficiency identified using 16S rRNA sequencing.

Case presentation:

A 35-year-old primipara and a 37-year-old nullipara were admitted to Hallym University Medical Center, Korea. They were negative for nitrazine and Actim PROM (Medix Biochemica) tests, and their amniotic membranes had prolapsed beyond the external os. A physical examination indicated that cerclage using a uniconcave balloon should be carried out, and amnioreduction was performed. Thereafter, they delivered at very early pre-term birth of below 24 weeks and the birth outcomes were pre-natal death. Analysis of mid-trimester amniotic fluids in these two pregnant women with cervical insufficiency identified and . These bacteria were not identified using vaginal or amniotic cultures.

Conclusion:

These results suggest that 16S rRNA gene sequencing can be used to identify the predominant microbiome causing pre-term birth in pregnant women with cervical insufficiency. Further studies are needed in large samples to improve our understanding of microbes causing pre-term birth and to prevent pre-term birth in pregnant women with cervical insufficiency.

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2016-04-19
2020-01-26
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References

  1. Baldwin E. A., Walther-Antonio M., MacLean A. M., Gohl D. M., Beckman K. B., Chen J., White B., Creedon D. J., Chia N.. 2015; Persistent microbial dysbiosis in preterm premature rupture of membranes from onset until delivery. PeerJ26:e1398 [CrossRef][PubMed]
    [Google Scholar]
  2. Bolstad A. I., Jensen H. B., Bakken V.. 1996; Taxonomy, biology, and periodontal aspects of Fusobacterium nucleatum. Clin Microbiol Rev9:55–71[PubMed]
    [Google Scholar]
  3. Cahill R. J., Tan S., Dougan G., O'Gaora P., Pickard D., Kennea N., Sullivan M. H., Feldman R. G., Edwards A. D., Universal D.N.A.. 2005; Universal DNA primers amplify bacterial DNA from human fetal membranes and link Fusobacterium nucleatum with prolonged preterm membrane rupture. Mol Hum Reprod11:761–766 [CrossRef][PubMed]
    [Google Scholar]
  4. Cao B., Stout M. J., Lee I., Mysorekar I. U.. 2014; Placental microbiome and its role in preterm birth. Neoreviews15:e537–e545 [CrossRef][PubMed]
    [Google Scholar]
  5. Collins M. D., Hoyles L., Tornqvist E., von Essen R., Falsen E.. 2001; Characterization of some strains from human clinical sources which resemble “Leptotrichia sanguinegens”: description of Sneathia sanguinegens sp. nov. gen. nov. Syst Appl Microbiol24:358–361 [CrossRef][PubMed]
    [Google Scholar]
  6. Coppenhagen-Glazer S., Sol A., Abed J., Naor R., Zhang X., Han Y. W., Bachrach G.. 2015; Fap2 of Fusobacterium nucleatum is a galactose-inhibitable adhesin involved in coaggregation, cell adhesion, and preterm birth. Infect Immun83:1104–1113 [CrossRef][PubMed]
    [Google Scholar]
  7. DiGiulio D. B.. 2012; Diversity of microbes in amniotic fluid. Semin Fetal Neonatal Med17:2–11 [CrossRef][PubMed]
    [Google Scholar]
  8. DiGiulio D. B., Romero R., Kusanovic J. P., Gómez R., Kim C. J., Seok K. S., Gotsch F., Mazaki-Tovi S., Vaisbuch E..Other authors 2010; Prevalence and diversity of microbes in the amniotic fluid, the fetal inflammatory response, and pregnancy outcome in women with preterm pre-labor rupture of membranes. Am J Reprod Immunol 64, 38-57[PubMed]
    [Google Scholar]
  9. Gardella C., Riley D. E., Hitti J., Agnew K., Krieger J. N., Eschenbach D.. 2004; Identification and sequencing of bacterial rDNAs in culture-negative amniotic fluid from women in premature labor. Am J Perinatol21:319–323 [CrossRef][PubMed]
    [Google Scholar]
  10. Gauthier S., Tétu A., Himaya E., Morand M., Chandad F., Rallu F., Bujold E.. 2011; The origin of Fusobacterium nucleatum involved in intra-amniotic infection and preterm birth. J Matern Fetal Neonatal Med24:1329–1332 [CrossRef][PubMed]
    [Google Scholar]
  11. Goto M., Hitomi S., Ishii T.. 2007; Bacterial arthritis caused by Leptotrichia amnionii. J Clin Microbiol45:2082–2083 [CrossRef][PubMed]
    [Google Scholar]
  12. Gravett M. G., Hummel D., Eschenbach D. A., Holmes K. K.. 1986; Preterm labor associated with subclinical amniotic fluid infection and with bacterial vaginosis. Obstet Gynecol67:229–237 [CrossRef][PubMed]
    [Google Scholar]
  13. Han Y. W., Redline R. W., Li M., Yin L., Hill G. B., McCormick T. S.. 2004; Fusobacterium nucleatum induces premature and term stillbirths in pregnant mice: implication of oral bacteria in preterm birth. Infect Immun72:2272–2279 [CrossRef][PubMed]
    [Google Scholar]
  14. Han Y. W., Shen T., Chung P., Buhimschi I. A., Buhimschi C. S.. 2009; Uncultivated bacteria as etiologic agents of intra-amniotic inflammation leading to preterm birth. J Clin Microbiol47:38–47 [CrossRef][PubMed]
    [Google Scholar]
  15. Han Y. W., Fardini Y., Chen C., Iacampo K. G., Peraino V. A., Shamonki J. M., Redline R. W.. 2010; Term stillbirth caused by oral Fusobacterium nucleatum. Obstet Gynecol115:442–445 [CrossRef][PubMed]
    [Google Scholar]
  16. Hassan S., Romero R., Hendler I., Gomez R., Khalek N., Espinoza J., Nien J. K., Berry S. M., Bujold E..Other authors 2006; A sonographic short cervix as the only clinical manifestation of intra-amniotic infection. J Perinat Med34:13–19 [CrossRef][PubMed]
    [Google Scholar]
  17. Hill G. B.. 1998; Preterm birth: associations with genital and possibly oral microflora. Ann Periodontol3:222–232 [CrossRef][PubMed]
    [Google Scholar]
  18. Hitti J., Hillier S. L., Agnew K. J., Krohn M. A., Reisner D. P., Eschenbach D. A.. 2001; Vaginal indicators of amniotic fluid infection in preterm labor. Obstet Gynecol97:211–219 [CrossRef][PubMed]
    [Google Scholar]
  19. Hyman R. W., Fukushima M., Jiang H., Fung E., Rand L., Johnson B., Vo K. C., Caughey A. B., Hilton J. F..Other authors 2014; Diversity of the vaginal microbiome correlates with preterm birth. Reprod Sci21:32–40 [CrossRef][PubMed]
    [Google Scholar]
  20. Lee S. E., Romero R., Park C. W., Jun J. K., Yoon B. H.. 2008; The frequency and significance of intraamniotic inflammation in patients with cervical insufficiency. Am J Obstet Gynecol198:e1–e8[PubMed]
    [Google Scholar]
  21. MacIntyre D. A., Chandiramani M., Lee Y. S., Kindinger L., Smith A., Angelopoulos N., Lehne B., Arulkumaran S., Brown R..other authors 2015; The vaginal microbiome during pregnancy and the postpartum period in a European population. Sci Rep11:8988 [CrossRef][PubMed]
    [Google Scholar]
  22. Moore W. E., Moore L. V.. 2000; The bacteria of periodontal disease. Periodontol5:66–77 [CrossRef]
    [Google Scholar]
  23. Romero R., Hassan S. S., Gajer P., Tarca A. L., Fadrosh D. W., Bieda J., Chaemsaithong P., Miranda J., Chaiworapongsa T., Ravel J.. 2014; The vaginal microbiota of pregnant women who subsequently have spontaneous preterm labor and delivery and those with a normal delivery at term. Microbiome2:18 [CrossRef][PubMed]
    [Google Scholar]
  24. Thilesen C. M., Nicolaidis M., Lökebö J. E., Falsen E., Jorde A. T., Müller F.. 2007; Leptotrichia amnionii, an emerging pathogen of the female urogenital tract. J Clin Microbiol45:2344–2347 [CrossRef][PubMed]
    [Google Scholar]
  25. Wang X., Buhimschi C. S., Temoin S., Bhandari V., Han Y. W., Buhimschi I. A.. 2013; Comparative microbial analysis of paired amniotic fluid and cord blood from pregnancies complicated by preterm birth and early-onset neonatal sepsis. PLoS One8:e56131 [CrossRef][PubMed]
    [Google Scholar]
  26. Wassenaar T. M., Panigrahi P.. 2014; Is a foetus developing in a sterile environment?. Lett Appl Microbiol59:572–579 [CrossRef][PubMed]
    [Google Scholar]
  27. Yoon B. H., Romero R., Moon J. B., Shim S. S., Kim M., Kim G., Jun J. K.. 2001; Clinical significance of intra-amniotic inflammation in patients with preterm labor and intact membranes. Am J Obstet Gynecol185:1130–1136 [CrossRef][PubMed]
    [Google Scholar]
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