| Bacterial strain or plasmid | Genotype or phenotype | Reference |
|---|---|---|
| P. aeruginosa | ||
| PAO1 | Wild-type | Dunn & Holloway (1971) |
| PAO9104 | narL : : cat, CmR | Krieger et al. (2002) |
| BB43 | PAO1 attB : : (P arcD -lacZ) | This work |
| BB45 | PAO9104 attB : : (P arcD -lacZ) | This work |
| BB46 | PAO1 attB : : (P arcDΔNarL -lacZ) | This work |
| E. coli | ||
| DH10B | F− mcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15 ΔlacX74 deoR recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ − rpsL nupG | GibcoBRL (Invitrogen) |
| S17 λ-pir | pro thi hsdR + Tpr Smr; chromosome : : RP4-2 Tc : : Mu-Km : : Tn7/λpir | de Lorenzo & Timmis (1994) |
| SM10 | thi-1 thr leu tonA lacY supE recA : : RP4-2-Tc : : Mu (KmR) | de Lorenzo & Timmis (1994) |
| Plasmids | ||
| mini-CTX-lacZ | TcR; promoterless lacZ gene | Becher & Schweizer (2000) |
| pFLP2 | ApR; source of FLP recombinase | Hoang et al. (1998) |
| pDH11 | TcR; mini-CTX-lacZ containing a 1005 bp PstI–BamHI fragment encoding the arcD promoter | Dana Heldt |
| pBB20 | TcR; pDH11 with the mutated NarL-binding site | This work |
Table 1.
Strains and plasmids used in this study
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