1887

Abstract

A Gram-stain-negative, rod-shaped bacterium, motile by means of a single polar flagellum, designated S-6-2, was isolated from petroleum polluted river sediment in Huangdao, Shandong Province, PR China. The 16S rRNA gene sequence analysis revealed that S-6-2 represented a member of the genus , sharing the highest sequence similarities with (97.5 %) and (97.5 %). Phylogenetic analysis based on 16S rRNA gene, concatenated 16S rRNA, , and genes and genome core-genes indicated that S-6-2 was affiliated with the members of the group. The average nucleotide identity (ANI) and genome-to-genome distance between the whole genome sequences of S-6-2 and closely related species of the genus within the group were less than 77.94 % and 20.5 %, respectively. Differences in phenotypic characteristics were also found between S-6-2 and the closely related species. The major cellular fatty acids (>10 %) were summed feature 8 (Cω7/ C ω6), C, Ccyclo and C. The predominant respiratory quinone was ubiquinone 9. The major polar lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), one unidentified lipid (L1), two unidentified phospholipids (PL1 and PL2) and an aminophospholipid (APL). The DNA G+C content of