1887

Abstract

has been reported as a destructive pathogen of Previous research suggested that the pathogenesis may not only be perpetuated by , but also by bacteria. Clarification of the interaction between and is a prerequisite for the development of effective control measures against wet bubble disease. Here, the effects of on mycelia are examined in dual culture on agar media and in open-ended test tubes. During disease development, the putative causal agents and cytology of wet bubble-diseased mushrooms were followed microscopically. The interaction between and the basidiome of was also studied using dual-cultured and basidiome tissues. Dual-cultured mycelia from both fungi showed that growth continued even after contact was made, without any observable antagonistic lines or cytoplasmic changes of mycelia. could be isolated from diseased basidiomes any time after inoculation, but bacteria were only recovered after the basidiomes of had been killed by . Dual culture of the basidiome tissue of and on agar media established that can independently and rapidly degrade the basidiomes of We conclude that has no pathogenic activity on the mycelial stage of but it can destroy basidiomes in the absence of bacteria. Wet bubble disease is evidently not caused by bacteria, but by the fungus, although bacteria likely participate in the disease after invasion by the fungus.

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2017-09-01
2024-03-29
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