1887

Abstract

SUMMARY: is the predominant microbial resident within the pilosebaceous follicles of sebum-rich areas of human skin. This study investigated the effects of known hydrophobic components of sebum on the physiology and nutrition of this microorganism, grown anaerobically at 33 °C, under defined conditions using continuous culture techniques. The medium used was chemically defined, comprising eight amino acids, with glucose as the main carbon energy source, and the culture pH was maintained at 5.6. The range of sebum lipids assayed was based on the C monounsaturated fatty acid 9--octadecenoic acid (oleic acid). Stock micronized solutions were aseptically pulsed into continuous cultures in the presence and absence of glucose, and nutritional effects monitored. None of the lipid substrates significantly affected growth either in terms of maximum specific growth rate (μ) or final culture biomass yield. Glycerol (3 mg ml) was found to be a poor carbon/energy source in comparison to glucose. Bacterial cells did, however, adhere with varying degrees, to the different lipid species, with maximum adherence occurring with the free fatty acid. This observation was confirmed by preliminary uptake experiments using [C]oleic acid. The interactive site for cell adherence may be the lipid-fibrillar layer associated with the cell surface of as discerned in electron microscopical studies. The findings of this investigation suggest that one function of the lipase may be to aid colonization within the pilosebaceous follicle, by promoting cell adherence to components such as oleic acid.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-139-8-1745
1993-08-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/139/8/mic-139-8-1745.html?itemId=/content/journal/micro/10.1099/00221287-139-8-1745&mimeType=html&fmt=ahah

References

  1. Bojar R.A., Holland K.T., Cunliffe W.J. 1991; Thein vitroantimicrobial effects of azelaic acid uponPropionibacterium acnesstrain P37. Journal of Antimicrobial Chemotherapy 28:843–853
    [Google Scholar]
  2. Cove J.H., Holland K.T., Cunliffe W.J. 1983; Effects of oxygen concentration on biomass production, maximum specific growth rate and extracellular enzyme production by three species of cutaneous propionibacteria grown in continuous culture.. Journal of General Microbiology 129:3327–3334
    [Google Scholar]
  3. Eaves G., Greenman J., Holland K.T. 1979; Decay rates of extracellular enzymes produced by bacteria-correction of production rates in chemostat cultures.. FEMS Microbiology Letters 6:333–336
    [Google Scholar]
  4. Ferguson D.A., Cummings C.S. 1978; Nutritonal requirements of anaerobic coryneforms.. Journal of Bacteriology 135:858–867
    [Google Scholar]
  5. Finnerty W.R. 1989; Microbial lipid metabolism.. In Microbial Lipids 2 pp. 525–566 Ratledge C., Wilkinson S.G. Edited by London: Academic Press;
    [Google Scholar]
  6. Greenman J., Holland K.T., Cunliffe W.J. 1981; Effects of glucose concentration on biomass, maximum specific growth rate and extracellular enzyme production by three species of cutaneous propionibacteria grown in continuous culture.. Journal of General Microbiology 127:371–376
    [Google Scholar]
  7. Greenman J., Holland K.T., Cunliffe W.J. 1983; Effects of pH on biomass, maximum specific growth rate and extracellular enzyme production by three specimens of cutaneous propionibacteria grown in continuous culture.. Journal of General Microbiology 129:1301–1307
    [Google Scholar]
  8. Holland K.T., Greenman J., Cunliffe W.J. 1979; Growth of cutaneous propionibacteria on synthetic medium; growth yields and exoenzyme production.. Journal of Applied Bacteriology 47:383–394
    [Google Scholar]
  9. Ingham E., Holland K.T., Gowland G., Cunliffe W.J. 1981; Partial purification and characterization of lipase (EC 3.1.1.3) fromPropionibacterium acnes. . Journal of General Microbiology 124:393–401
    [Google Scholar]
  10. Kellum R.E. 1967; Human sebaceous gland lipids.. Archives of Dermatology 95:218–220
    [Google Scholar]
  11. Ko H.L., Heczko P.B., Pulverer G. 1978; Differential susceptibility ofPropionibacterium Acnes P.granulosumandP. avidumto free fatty acids.. Journal of Investigative Dermatology 71:363–365
    [Google Scholar]
  12. Leeming J.P., Holland K.T., Cunliffe W.J. 1984; The microbial ecology of pilosebaceous units isolated from human skin.. Journal of General Microbiology 130:803–807
    [Google Scholar]
  13. Leeming J.P., Holland K.T., Cunliffe W.J. 1985; The pathological and ecological significance of microorganisms colonising acne vulgaris comedones.. Journal of Medical Microbiology 20:11–16
    [Google Scholar]
  14. Marples R.R., Mcginley K.J. 1974; Corynebacterium acnesand other anaerobic diphtheroids from human skin.. Journal of Medical Microbiology 7:349–357
    [Google Scholar]
  15. Marples R.R., Downing D.T., Kligman A.M. 1971; Control of free fatty acids in human surface lipids byCorynebacterium acnes. . Journal of Investigative Dermatology 56:127–131
    [Google Scholar]
  16. Mcginley K.J., Webster G.F., Leyden J.J. 1978; Regional variations of cutaneous propionibacteria.. Applied and Environmental Microbiology 35:62–66
    [Google Scholar]
  17. Noble W.C. 1981; Nutrient factors.. In Microbiology of Human Skin pp. 26–65 Rook A. Edited by London: Lloyd-Luke Ltd.;
    [Google Scholar]
  18. Pirt S.J. 1975 Principles of Microbe and Cell cultivation. Oxford: Blackwell Scientific Publications.;
    [Google Scholar]
  19. Puhvel S.M., Reisner R.M. 1970; Effect of fatty acids on the growth of Corynebacterium acnes in vitro.. Journal of Investigative Dermatology 54:48–52
    [Google Scholar]
  20. Rebello T., Hawk J.L.M. 1978; Skin surface glycerol levels in acne vulgaris.. Journal of Investigative Dermatology 70:352–354
    [Google Scholar]
  21. Stromberg N., Karlsson K.A. 1990; Characterization of the binding ofPropionibacterium granulosumto glycosphingolipids adsorbed on surfaces.. Journal of Biological Chemistry 265:11244–11250
    [Google Scholar]
  22. Summerly R., Woodbury S. 1971; Thein vitroincorporation of [l4C]acetate into the isolated sebaceous glands and appendage-freed epidermis of human skin. A technique for the study of lipid synthesis in the isolated sebaceous gland.. British Journal of Dermatology 85:424–431
    [Google Scholar]
  23. Ushijima T., Takahashi M., Ozaki Y. 1984; Acetic, propionic and oleic acid as the possible factorsinfluencing the predominant residence of some species ofPropionibacteriumand coagulase-negativeStaphylococcuson normal skin.. Canadian Journal of Microbiology 30:647–652
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-139-8-1745
Loading
/content/journal/micro/10.1099/00221287-139-8-1745
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error