1887

Abstract

Non-thermal plasma (NTP) is a flow of partially ionized argon gas at an ambient macroscopic temperature and is microbicidal for bacteria, viruses and fungi. Viability of the Gram-negative obligate intracellular bacterial parasite and its host cells was investigated after NTP treatment. NTP treatment of extracellular elementary bodies (EBs) diminished the concentration of infectious bacteria by a factor of 9×10, as established by the parallel infection of murine fibroblast McCoy cells with treated and control EBs. NTP treatment of infected McCoy cells caused disruption of membrane-restricted vacuoles (inclusions), where intracellular reticulate bodies (RBs) multiply, and a 2×10-fold reduction in the concentration of infectious bacteria. When the samples were covered with magnesium fluoride glass to obstruct plasma particles and UV rays alone were applied, the bactericidal effect was reduced 1.4×10-fold and 5×10-fold for EBs and RBs, respectively. NTP treatment caused the viability of host McCoy cells to diminish by 19 %. Therefore, the results obtained demonstrated that (i) both extracellular and intracellular forms of are sensitive to NTP treatment; (ii) the reduction in concentration of infectious bacteria after NTP treatment of infected cells is superior to the reduction in viability of host cells; and (iii) the effect of NTP on intracellular bacteria does not depend on UV rays.

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2012-06-01
2024-03-29
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References

  1. Adderley-Kelly B., Stephens E. M. 2005; Chlamydia: a major health threat to adolescents and young adults. ABNF J 16:52–55[PubMed]
    [Google Scholar]
  2. Beagley K. W., Timms P. 2000; Chlamydia trachomatis infection: incidence, health costs and prospects for vaccine development. J Reprod Immunol 48:47–68 [View Article][PubMed]
    [Google Scholar]
  3. Bébéar C., de Barbeyrac B. 2009; Genital Chlamydia trachomatis infections. Clin Microbiol Infect 15:4–10 [View Article][PubMed]
    [Google Scholar]
  4. Byrne G. I., Ojcius D. M. 2004; Chlamydia and apoptosis: life and death decisions of an intracellular pathogen. Nat Rev Microbiol 2:802–808 [View Article][PubMed]
    [Google Scholar]
  5. Caldwell H. D., Kromhout J., Schachter J. 1981; Purification and partial characterization of the major outer membrane protein of Chlamydia trachomatis. Infect Immun 31:1161–1176[PubMed]
    [Google Scholar]
  6. Carabeo R. A., Grieshaber S. S., Fischer E., Hackstadt T. 2002; Chlamydia trachomatis induces remodeling of the actin cytoskeleton during attachment and entry into HeLa cells. Infect Immun 70:3793–3803 [View Article][PubMed]
    [Google Scholar]
  7. Cocchiaro J. L., Valdivia R. H. 2009; New insights into Chlamydia intracellular survival mechanisms. Cell Microbiol 11:1571–1578 [View Article][PubMed]
    [Google Scholar]
  8. Dautry-Varsat A., Balañá M. E., Wyplosz B. 2004; Chlamydia–host cell interactions: recent advances on bacterial entry and intracellular development. Traffic 5:561–570 [View Article][PubMed]
    [Google Scholar]
  9. Dobrynin D., Fridman G., Friedman G., Fridman A. 2009; Physical and biological mechanisms of direct plasma interaction with living tissue. New J Phys 11:115020 [View Article]
    [Google Scholar]
  10. Dong F., Pirbhai M., Xiao Y., Zhong Y., Wu Y., Zhong G. 2005; Degradation of the proapoptotic proteins Bik, Puma, and Bim with Bcl-2 domain 3 homology in Chlamydia trachomatis-infected cells. Infect Immun 73:1861–1864 [View Article][PubMed]
    [Google Scholar]
  11. Ermolaeva S. A., Varfolomeev A. F., Chernukha M. Y., Yurov D. S., Vasiliev M. M., Kaminskaya A. A., Moisenovich M. M., Romanova J. M., Murashev A. N. other authors 2011; Bactericidal effects of non-thermal argon plasma in vitro, in biofilms and in the animal model of infected wounds. J Med Microbiol 60:75–83 [View Article][PubMed]
    [Google Scholar]
  12. Fields K. A., Hackstadt T. 2002; The chlamydial inclusion: escape from the endocytic pathway. Annu Rev Cell Dev Biol 18:221–245 [View Article][PubMed]
    [Google Scholar]
  13. Fricke K., Steffen H., von Woedtke T., Schröder K., Weltmann K.-D. 2011; High rate etching of polymers by means of an atmospheric pressure plasma jet. Plasma Process Polym 8:51–58 [View Article]
    [Google Scholar]
  14. Hackstadt T., Fischer E. R., Scidmore M. A., Rockey D. D., Heinzen R. A. 1997; Origins and functions of the chlamydial inclusion. Trends Microbiol 5:288–293 [View Article][PubMed]
    [Google Scholar]
  15. Hammerschlag M. R. 2002; The intracellular life of Chlamydiae. Semin Pediatr Infect Dis 13:239–248 [View Article][PubMed]
    [Google Scholar]
  16. Isbary G., Morfill G., Schmidt H. U., Georgi M., Ramrath K., Heinlin J., Karrer S., Landthaler M., Shimizu T. other authors 2010; A first prospective randomized controlled trial to decrease bacterial load using cold atmospheric argon plasma on chronic wounds in patients. Br J Dermatol 163:78–82[PubMed]
    [Google Scholar]
  17. Joaquin J. C., Kwan C., Abramzon N., Vandervoort K., Brelles-Mariño G. 2009; Is gas-discharge plasma a new solution to the old problem of biofilm inactivation?. Microbiology 155:724–732 [View Article][PubMed]
    [Google Scholar]
  18. Joshi S. G., Paff M., Friedman G., Fridman G., Fridman A., Brooks A. D. 2010; Control of methicillin-resistant Staphylococcus aureus in planktonic form and biofilms: a biocidal efficacy study of nonthermal dielectric-barrier discharge plasma. Am J Infect Control 38:293–301 [View Article][PubMed]
    [Google Scholar]
  19. Kalghatgi S., Friedman G., Fridman A., Clyne A. M. 2010; Endothelial cell proliferation is enhanced by low dose non-thermal plasma through fibroblast growth factor-2 release. Ann Biomed Eng 38:748–757 [View Article][PubMed]
    [Google Scholar]
  20. Kalghatgi S., Kelly C. M., Cerchar E., Torabi B., Alekseev O., Fridman A., Friedman G., Azizkhan-Clifford J. 2011; Effects of non-thermal plasma on mammalian cells. PLoS ONE 6:e16270 [View Article][PubMed]
    [Google Scholar]
  21. Kayes M. M., Critzer F. J., Kelly-Wintenberg K., Roth J. R., Montie T. C., Golden D. A. 2007; Inactivation of foodborne pathogens using a one atmosphere uniform glow discharge plasma. Foodborne Pathog Dis 4:50–59 [View Article][PubMed]
    [Google Scholar]
  22. King L. E. J. Jr, Stratton C. W., Mitchell W. M. 2001; Chlamydia pneumoniae and chronic skin wounds: a focused review. J Investig Dermatol Symp Proc 6:233–237 [View Article][PubMed]
    [Google Scholar]
  23. Kolb J. F., Kong M. G., Blackmore P. F. 2006; Special issue on nonthermal medical/biological applications using ionized gases and electromagnetic fields. IEEE Trans Plasma Sci 34:1250–1252 [View Article]
    [Google Scholar]
  24. Kong M., Kroesen G., Morfill G., Nosenko T., Shimizu T., van Dijk J., Zimmermann J. L. 2009; Plasma medicine: an introductory review. New J Phys 11:115012 [View Article]
    [Google Scholar]
  25. Laroussi M. 2005; Low temperature plasma-based sterilization: overview and state-of-the-art. Plasma Process Polym 2:391–400 [View Article]
    [Google Scholar]
  26. Laroussi M., Mendis D., Rosenberg M. 2003; Plasma interaction with microbes. New J Phys 5:41.1–41.10 [View Article]
    [Google Scholar]
  27. Leduc M., Guay D., Leask R. L., Coulombe S. 2009; Cell permeabilization using a non-thermal plasma. New J Phys 11:115021–115029 [View Article]
    [Google Scholar]
  28. Lee K., Paek K. H., Ju W. T., Lee Y. 2006; Sterilization of bacteria, yeast, and bacterial endospores by atmospheric-pressure cold plasma using helium and oxygen. J Microbiol 44:269–275[PubMed]
    [Google Scholar]
  29. Moreau M., Orange N., Feuilloley M. G. J. 2008; Non-thermal plasma technologies: new tools for bio-decontamination. Biotechnol Adv 26:610–617 [View Article][PubMed]
    [Google Scholar]
  30. Nosenko T., Shimizu T., Morfill G. E. 2009; Designing plasmas for chronic wound disinfection. New J Phys 11:115013 [View Article]
    [Google Scholar]
  31. Robert E., Barbosa E., Dozias S., Vandamme M., Cachoncinlle C., Viladrosa R., Pouvesle J. M. 2009; Experimental study of a compact nanosecond plasma gun. Plasma Process Polym 6:795–802
    [Google Scholar]
  32. Sensenig R., Kalghatgi S., Cerchar E., Fridman G., Shereshevsky A., Torabi B., Arjunan K. P., Podolsky E., Fridman A. other authors 2011; Non-thermal plasma induces apoptosis in melanoma cells via production of intracellular reactive oxygen species. Ann Biomed Eng 39:674–687 [View Article][PubMed]
    [Google Scholar]
  33. Shimizu T., Steffes B., Pompl R., Jamitzky F., Bunk W., Ramrath K., Georgi M., Stolz W., Schmidt H. other authors 2008; Characterization of microwave plasma torch for decontamination. Plasma Process Polym 5:577–582 [View Article]
    [Google Scholar]
  34. Venezia R. A., Orrico M., Houston E., Yin S. M., Naumova Y. Y. 2008; Lethal activity of nonthermal plasma sterilization against microorganisms. Infect Control Hosp Epidemiol 29:430–436 [View Article][PubMed]
    [Google Scholar]
  35. Volotskova O., Shashurin A., Stepp M., Pal-Ghosh S., Keidar M. 2011; Plasma-controlled cell migration: localization of cold plasma–cell interaction region. Plasma Med 1:85–92 [View Article]
    [Google Scholar]
  36. Yasin B., Harwig S. S., Lehrer R. I., Wagar E. A. 1996; Susceptibility of Chlamydia trachomatis to protegrins and defensins. Infect Immun 64:709–713[PubMed]
    [Google Scholar]
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