1887

Abstract

To better understand genomes and virulence, microarray comparative genome hybridization (mCGH) data were collected from one , two , two and 48 isolates. For , these isolates are from diverse clonal complexes, invasive and carriage strains, and all major serogroups. The microarray platform represented strains MC58, Z2491 and FAM18, and FA1090. By comparing hybridization data to genome sequences, the core genome and insertions/deletions (e.g. capsule locus, type I secretion system) related to pathogenicity were identified, including further characterization of the capsule locus, bioinformatics analysis of a type I secretion system, and identification of some metabolic pathways associated with intracellular survival in pathogens. Hybridization data clustered meningococcal isolates from similar clonal complexes that were distinguished by the differential presence of six distinct islands of horizontal transfer. Several of these islands contained prophage or other mobile elements, including a novel prophage and a transposon carrying portions of a type I secretion system. Acquisition of some genetic islands appears to have occurred in multiple lineages, including transfer between and . However, island acquisition occurs infrequently, such that the genomic-level relationship is not obscured within clonal complexes. The genome is characterized by the horizontal acquisition of multiple genetic islands; the study of these islands reveals important sets of genes varying between isolates and likely to be related to pathogenicity.

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2006-12-01
2019-11-12
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mCGH results for the capsule region of each strain examined. mCGH results demonstrate different configurations in the capsule region. The results are mapped to the MC58 genome (red, absent/divergent; blue, not predictive; white; present) and unique regions of the other capsule regions as illustrated in Fig. 5 of the main paper. The bottom line indicates colour codes from panel A of Fig. 5. [ PDF] (858 kb) Average log (Ratio) for each locus for each strain examined. [ Excel file] (2927 kb) Summary of all the gene categorizations in this study as well as selected previous genomics studies [ Excel file] (385 kb)

PDF

mCGH results for the capsule region of each strain examined. mCGH results demonstrate different configurations in the capsule region. The results are mapped to the MC58 genome (red, absent/divergent; blue, not predictive; white; present) and unique regions of the other capsule regions as illustrated in Fig. 5 of the main paper. The bottom line indicates colour codes from panel A of Fig. 5. [ PDF] (858 kb) Average log (Ratio) for each locus for each strain examined. [ Excel file] (2927 kb) Summary of all the gene categorizations in this study as well as selected previous genomics studies [ Excel file] (385 kb)

EXCEL

mCGH results for the capsule region of each strain examined. mCGH results demonstrate different configurations in the capsule region. The results are mapped to the MC58 genome (red, absent/divergent; blue, not predictive; white; present) and unique regions of the other capsule regions as illustrated in Fig. 5 of the main paper. The bottom line indicates colour codes from panel A of Fig. 5. [ PDF] (858 kb) Average log (Ratio) for each locus for each strain examined. [ Excel file] (2927 kb) Summary of all the gene categorizations in this study as well as selected previous genomics studies [ Excel file] (385 kb)

EXCEL
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