1887

Abstract

Stable tetracycline resistance in is mediated by a family of genomic islands [the (C) islands] that are integrated into the chlamydial chromosome. The (C) islands contain several plasmid-specific genes, the (C) resistance gene and, in most cases, a novel insertion element (IS) encoding two predicted transposases. The hypothesis that IS mediated the integration of the (C) resistance islands into the genome was tested using a plasmid-based transposition system in . Both high- and medium-copy-number plasmids were used as carriers of IS in these experiments. IS integrated into a target plasmid (pOX38) when delivered by either donor plasmid, and integration of the entire donor plasmid was common. IS-mediated integration occurred at many positions within pOX38, with 36 of 38 events adjacent to a 5′-TTCAA-3′ sequence. Deletions in each of the candidate transposase genes within IS demonstrated that only one of the two ORFs was necessary for the observed transposition activity and target specificity. Analysis of progeny from the mating assays also indicated that IS can excise following integration into a target DNA, and, in each tested case, the sequence 5′-AATTCAA-3′ remained at the site of excision. Collectively, these results are consistent with the nucleotide sequence data collected for the (C) islands, and strongly suggest that a transposase within IS is responsible for integration of these genomic islands into the chromosome.

Keyword(s): IS, insertion element
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2007-01-01
2020-07-16
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