1887

Abstract

The ecophysiology of five filamentous species affiliated to the was investigated in industrial activated sludge systems. The five species, ‘ Alysiosphaera europaea’, ‘ Monilibacter batavus’, ‘ Alysiomicrobium bavaricum’, ‘ Sphaeronema italicum’ and , are very abundant in industrial wastewater treatment plants and are often involved in bulking incidents. The morphology of these filamentous bacterial species resembled Eikelboom's , or Type 021N, and could only be correctly identified by using fluorescence hybridization (FISH), applying species-specific gene probes. Two physiological groupings of the five species were found using microautoradiography combined with FISH. Group 1 (‘ Monilibacter batavus' and ‘ Sphaeronema italicum’) utilized many short-chained fatty acids (acetate, pyruvate and propionate), whereas Group 2 (‘ Alysiosphaera europaea’, ‘ Alysiomicrobium bavaricum’ and ) could also exploit several sugars, amino acids and ethanol. All species had polyhydroxyalkanoate granules present and several of the species had a very large storage capacity. No activity was found under strict anaerobic conditions, while uptake of substrate was observed in the presence of nitrate or nitrite as potential electron acceptor. However, for all species a reduced number of substrates could be consumed under these conditions compared to aerobic conditions. Only a little exo-enzymic activity was found and nearly all species had a hydrophobic cell surface. Based on knowledge of the ecophysiological potential, control strategies are suggested.

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2006-10-01
2019-11-20
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References

  1. Amann, R. I. ( 1995; ). In situ identification of micro-organisms by whole cell hybridization with rRNA-targeted nucleic acid probes. In Molecular Microbial Ecological Manual, pp. 1–15. Edited by A. D. L. Akkermans, J. D. van Elsas & F. J. de Bruijn. London: Kluwer.
  2. Amann, R. I., Binder, B. J., Olson, R. J., Chisholm, S. W., Devereux, R. & Stahl, D. A. ( 1990; ). Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations. Appl Environ Microbiol 56, 1919–1925.
    [Google Scholar]
  3. Andreasen, K. & Nielsen, P. H. ( 2000; ). Growth of Microthrix parvicella in nutrient removal activated sludge plants: studies of in situ physiology. Water Res 34, 1559–1569.[CrossRef]
    [Google Scholar]
  4. Behrens, S., Ruhland, C., Inacio, J., Huber, H., Fonseca, A., Spencer-Martins, I., Fuchs, B. M. & Amann, R. ( 2003; ). In situ accessibility of small-subunit rRNA of members of the domains Bacteria, Archaea, and Eucarya to Cy3-labeled oligonucleotide probes. Appl Environ Microbiol 69, 1748–1758.[CrossRef]
    [Google Scholar]
  5. Daims, H., Bruhl, A., Amann, R., Schleifer, K. H. & Wagner, M. ( 1999; ). The domain-specific probe EUB338 is insufficient for the detection of all bacteria: development and evaluation of a more comprehensive probe set. Syst Appl Microbiol 22, 434–444.[CrossRef]
    [Google Scholar]
  6. Dawes, E. A. ( 1991; ). Storage polymers in prokaryotes. In Prokaryotic Structure and Function, pp. 81–122. Edited by S. Mohan, C. Dow & J. A. Coles. Cambridge: Cambridge University Press.
  7. Dionisi, D., Levantesi, C., Renzi, V., Tandoi, V. & Majone, M. ( 2002; ). PHA storage from several substrates by different morphological types in an anoxic/aerobic SBR. Water Sci Technol 46, 337–344.
    [Google Scholar]
  8. Eikelboom, D. H. ( 2002; ). Process Control of Activated Sludge Plants by Microscopic Investigation. London: IWA Publishing.
  9. Eikelboom, D. H. & Geurkink, B. ( 2002; ). Filamentous micro-organisms observed in industrial activated sludge plants. Water Sci Technol 46, 535–542.
    [Google Scholar]
  10. Eikelboom, D. H. & van Buijsen, H. J. ( 1983; ). Microscopic Sludge Investigation Manual, 2nd edn. Delft: TNO Research Institute for Environmental Hygiene.
  11. Foss, S. & Harder, J. ( 1998; ). Thauera linaloolentis sp. nov. and Thauera terpenica sp. nov., isolated on oxygen-containing monoterpenes (linalool, menthol, and eucalyptol) nitrate. Syst Appl Microbiol 21, 365–373.[CrossRef]
    [Google Scholar]
  12. Haywood, G. W., Anderson, A. J., Ewing, D. F. & Dawes, E. A. ( 1990; ). Accumulation of a polyhydroxyalkanoate containing primarily 3-hydroxydecanoate from simple carbohydrate substrates by Pseudomonas sp. strain NCIMB 40135. Appl Environ Microbiol 56, 3354–3359.
    [Google Scholar]
  13. Haywood, G. W., Anderson, A. J., Williams, D. R., Dawes, E. A. & Ewing, D. F. ( 1991; ). Accumulation of a poly(hydroxyalkanoate) copolymer containing primarily 3-hydroxyvalerate from simple carbohydrate substrates by Rhodococcus sp. NCIMB 40126. Int J Biol Macromol 13, 83–88.[CrossRef]
    [Google Scholar]
  14. Jenkins, D., Richard, M. & Daigger, G. ( 1993; ). Manual on Cases and Control of Activated Sludge Bulking and Foaming. Chelsea, MI: Lewis Publishers.
  15. Jenkins, D., Richard, M. G. & Daigger, G. T. ( 2004; ). Manual on the Causes and Control of Activated Sludge Bulking, Foaming, and Other Solids Separation Problems. London: IWA Publishing, CRC Press.
  16. Kanagawa, T., Kamagata, Y., Aruga, S., Kohno, T., Horn, M. & Wagner, M. ( 2000; ). Phylogenetic analysis of and oligonucleotide probe development for eikelboom type 021N filamentous bacteria isolated from bulking activated sludge. Appl Environ Microbiol 66, 5043–5052.[CrossRef]
    [Google Scholar]
  17. Kong, Y. H., Nielsen, J. L. & Nielsen, P. H. ( 2004; ). Microautoradiographic study of Rhodocyclus-related polyphosphate accumulating bacteria in full-scale enhanced biological phosphorus removal plants. Appl Environ Microbiol 70, 5383–5390.[CrossRef]
    [Google Scholar]
  18. Kong, Y. H., Xia, Y., Nielsen, J. L. & Nielsen, P. H. ( 2006; ). Ecophysiology of a group of uncultured Gammaproteobacterial glycogen-accumulating organisms in full scale EBPR wastewater treatment plants. Environ Microbiol 8, 479–489.[CrossRef]
    [Google Scholar]
  19. Kragelund, C., Nielsen, J. L., Thomsen, T. R. & Nielsen, P. H. ( 2005; ). Ecophysiology of the filamentous Alphaproteobacterium Meganema perideroedes in activated sludge. FEMS Microbiol Ecol 54, 111–122.[CrossRef]
    [Google Scholar]
  20. Lee, N., Nielsen, P. H., Andreasen, K. H., Juretschko, S., Nielsen, J. L., Schleifer, K. H. & Wagner, M. ( 1999; ). Combination of fluorescent in situ hybridization and microautoradiography – a new tool for structure–function analyses in microbial ecology. Appl Environ Microbiol 65, 1289–1297.
    [Google Scholar]
  21. Levantesi, C., Beimfohr, C., Geurkink, B., Rossetti, S., Thelen, K., Krooneman, J., Snaidr, J., van der Waarde, J. & Tandoi, V. ( 2004; ). Filamentous Alphaproteobacteria associated with bulking in industrial wastewater treatment plants. Syst Appl Microbiol 27, 716–727.[CrossRef]
    [Google Scholar]
  22. Loy, A., Horn, M. & Wagner, M. ( 2003; ). probeBase: an online resource for rRNA-targeted oligonucleotide probes. Nucleic Acids Res 31, 514–516.[CrossRef]
    [Google Scholar]
  23. Ludwig, W., Strunk, O., Westram, R., Richter, L. & 29 other authors ( 2004; ). arb: a software environment for sequence data. Nucleic Acids Res 32, 1363–1371.[CrossRef]
    [Google Scholar]
  24. Martins, A. M., Pagilla, K., Heijnen, J. J. & Van Loosdrecht, M. C. ( 2004; ). Filamentous bulking sludge – a critical review. Water Res 38, 793–817.[CrossRef]
    [Google Scholar]
  25. Neef, A. ( 1997; ). Anwendung der in situ-Einzelzell-Identifizierung von Bakterien zur Populationsanalyse in komplexen mikrobiellen Biozönose. PhD thesis, TU München, Germany.
  26. Nielsen, J. L., Mikkelsen, L. H. & Nielsen, P. H. ( 2001; ). In situ detection of cell surface hydrophobicity of probe-defined bacteria in activated sludge. Water Sci Technol 43, 97–103.
    [Google Scholar]
  27. Nielsen, P. H., Roslev, P., Dueholm, T. E. & Nielsen, J. L. ( 2002; ). Microthrix parvicella, a specialized lipid consumer in anaerobic–aerobic activated sludge plants. Water Sci Technol 46, 73–80.
    [Google Scholar]
  28. Ostle, A. G. & Holt, J. G. ( 1982; ). Nile blue A as a fluorescent stain for poly-β-hydroxybutyrate. Appl Environ Microbiol 44, 238–241.
    [Google Scholar]
  29. Shao, Y. J. & Jenkins, D. ( 1989; ). The use of anoxic selectors for the control of low F/M activated-sludge bulking. Water Sci Technol 21, 609–619.
    [Google Scholar]
  30. Snaidr, J., Beimfohr, C., Levantesi, C., Rossetti, S., van der Waarde, J., Geurkink, B., Elkelboom, D., Lemaitre, M. & Tandoi, V. ( 2002; ). Phylogenetic analysis and in situ identification of ‘Nostocoida limicola’-like filamentous bacteria in activated sludge from industrial wastewater treatment plants. Water Sci Technol 46, 99–104.
    [Google Scholar]
  31. Thomsen, T. R., Blackall, L. L., de Muro, M. A., Nielsen, J. L. & Nielsen, P. H. ( 2006; ). Meganema perideroedes gen. nov., sp. nov., a new filamentous Alphaproteobacterium from activated sludge. Int J Syst Evol Microbiol 56, 1865–1868.[CrossRef]
    [Google Scholar]
  32. van der Waarde, J., Krooneman, J., Geurkink, B., van der Werf, A., Eikelboom, D., Beimfohr, C., Snaidr, J., Levantesi, C. & Tandoi, V. ( 2002; ). Molecular monitoring of bulking sludge in industrial wastewater treatment plants. Water Sci Technol 46, 551–558.
    [Google Scholar]
  33. Wagner, M., Assmus, B., Hartmann, A., Hutzler, P. & Amann, R. ( 1994; ). In situ analysis of microbial consortia in activated sludge using fluorescently labelled, rRNA-targeted oligonucleotide probes and confocal scanning laser microscopy. J Microsc 176, 181–187.[CrossRef]
    [Google Scholar]
  34. Wanner, J. ( 1994; ). Activated Sludge Bulking and Foaming Control. Basel, Switzerland: Technomic Publishing Company.
  35. Zita, A. & Hermansson, M. ( 1997a; ). Determination of bacterial cell surface hydrophobicity of single cells in cultures and in wastewater in situ. FEMS Microbiol Lett 152, 299–306.[CrossRef]
    [Google Scholar]
  36. Zita, A. & Hermansson, M. ( 1997b; ). Effects of bacterial cell surface structures and hydrophobicity on attachment to activated sludge flocs. Appl Environ Microbiol 63, 1168–1170.
    [Google Scholar]
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