1887

Abstract

The ecophysiology of five filamentous species affiliated to the was investigated in industrial activated sludge systems. The five species, ‘ Alysiosphaera europaea’, ‘ Monilibacter batavus’, ‘ Alysiomicrobium bavaricum’, ‘ Sphaeronema italicum’ and , are very abundant in industrial wastewater treatment plants and are often involved in bulking incidents. The morphology of these filamentous bacterial species resembled Eikelboom's , or Type 021N, and could only be correctly identified by using fluorescence hybridization (FISH), applying species-specific gene probes. Two physiological groupings of the five species were found using microautoradiography combined with FISH. Group 1 (‘ Monilibacter batavus' and ‘ Sphaeronema italicum’) utilized many short-chained fatty acids (acetate, pyruvate and propionate), whereas Group 2 (‘ Alysiosphaera europaea’, ‘ Alysiomicrobium bavaricum’ and ) could also exploit several sugars, amino acids and ethanol. All species had polyhydroxyalkanoate granules present and several of the species had a very large storage capacity. No activity was found under strict anaerobic conditions, while uptake of substrate was observed in the presence of nitrate or nitrite as potential electron acceptor. However, for all species a reduced number of substrates could be consumed under these conditions compared to aerobic conditions. Only a little exo-enzymic activity was found and nearly all species had a hydrophobic cell surface. Based on knowledge of the ecophysiological potential, control strategies are suggested.

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2006-10-01
2020-10-21
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