1887

Abstract

The sigma gene, , of belongs to the group IV heat-shock response genes and has many orthologues in the bacterial phylum Firmicutes. The gene is considered to constitute an operon with (egulation of , formerly ). As little is known about either the structure and function of the operon or the SigI regulons, the role of RsgI in heat-inducible transcription of the operon was investigated, using Northern analysis and a heat-stable -galactosidase reporter assay. Heat-inducible, SigI-dependent transcription of the operon was stimulated greatly by disrupting . Yeast two-hybrid analysis showed direct interaction between the N-terminal portion of the presumed RsgI protein and SigI. Without RsgI function, induction of transcription of the operon upon transient heat stress depended on activity. However, transcription of the operon was induced during growth at prolonged higher temperature even without DnaK function. Without RsgI function, operon transcription was induced after the end of growth independent of any temperature shift in a sporulation medium and toward the end of growth in a rich complex medium. Furthermore, glucose addition resulted in a strong suppression of transcription. Therefore it is hypothesized that transcription of the operon of is negatively regulated by the putative transmembrane protein RsgI, which moderates SigI's sensitivity to heat shock or nutritional stress.

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2007-01-01
2020-07-16
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