1887

Abstract

causes symptoms of acute inflammatory diarrhoea in man. interaction with epithelial cells elicits interleukin-8 (IL-8) production, and IL-8 recruits neutrophils to sites of infection. Cell culture models of bacterial interaction with epithelium are useful to define bacteria–host interaction and are used because it is thought they mimic the same bacteria–host cell interaction in the natural disease. This study looks at the ability of strains to elicit IL-8 production from a variety of cell lines previously used for investigating the interaction of with host cells. A spectrum of IL-8 responses was observed, with minimal IL-8 elicited from Caco-2 cells and more marked responses elicited from HeLa and T84 cells. These -infected cell line responses were compared to IL-8 production from -infected human colonic and ileal tissue. The -infected tissue elicited the highest IL-8 responses and the cytokine was manifested earlier compared to the infected cell lines.

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2006-12-01
2020-07-08
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References

  1. Al-Salloom F. S, Al Mahmeed A, Ismaeel A, Botta G. A, Bakhiet M. 2003; Campylobacter-stimulated INT407 cells produce dissociated cytokine profiles. J Infect47:217–224[CrossRef]
    [Google Scholar]
  2. Bakhiet M, Al-Salloom F. S, Qareiballa A, Bindayna K, Farid I, Botta G. A. 2004; Induction of alpha and beta chemokines by intestinal epithelial cells stimulated with Campylobacter jejuni . J Infect48:236–244[CrossRef]
    [Google Scholar]
  3. Everest P. 2005; Campylobacter jejuni and intestinal inflammation. In Campylobacter, Molecular and Cellular Biology pp. 421–434 Edited by Ketley J., Konkel M.. Norwich: Horizon Press;
  4. Everest P. H, Goossens H, Butzler J. P, Lloyd D, Knutton S, Ketley J. M, Williams P. H. 1992; Differentiated Caco-2 cells as a model for enteric invasion by Campylobacter jejuni and C. coli . J Med Microbiol37:319–325[CrossRef]
    [Google Scholar]
  5. Everest P. H, Goossens H, Sibbons P, Lloyd D, Knutton S, Leece R, Ketley J. M, Williams P. H. 1993a; Pathological changes in the rabbit ileal loop model caused by Campylobacter jejuni from human colitis. J Med Microbiol38:316–321[CrossRef]
    [Google Scholar]
  6. Everest P. H, Cole A. T, Hawkey C. J, Goossens H, Knutton S, Butzler J. P, Ketley J. M, Williams P. H. 1993b; Roles of leukotriene B4, prostaglandin E2 and cyclic AMP in Campylobacter jejuni -induced intestinal fluid secretion. Infect Immun61:4885–4887
    [Google Scholar]
  7. Harvey P, Battle T, Leach S. 1999; Different invasion phenotypes of Campylobacter isolates in Caco-2 cell monolayers. J Med Microbiol48:461–469[CrossRef]
    [Google Scholar]
  8. Hickey T. E, Baqar S, Bourgeois A. L, Guerry P. 1999; Campylobacter jejuni stimulated secretion of interleukin-8 by INT-407 cells. Infect Immun67:88–93
    [Google Scholar]
  9. Hickey T. E, McVeigh A. L, Scott D. A, Michielutti R. E, Bixby A, Carroll S. A, Bourgeois A. L, Guerry P. 2000; Campylobacter jejuni cytolethal distending toxin mediates release of interleukin-8 from intestinal epithelial cells. Infect Immun68:6535–6541[CrossRef]
    [Google Scholar]
  10. Hickey T. E, Majam G, Guerry P. 2005; Intracellular survival of Campylobacter jejuni in human monocytic cells and induction of apoptotic death by cytolethal distending toxin. Infect Immun73:5194–5197[CrossRef]
    [Google Scholar]
  11. Hu L, Hickey T. E. 2005; Campylobacter jejuni induces secretion of proinflammatory chemokines from human intestinal epithelial cells. Infect Immun73:4437–4440[CrossRef]
    [Google Scholar]
  12. Ketley J. M. 1997; Pathogenesis of enteric infection by Campylobacter . Microbiology143:5–21[CrossRef]
    [Google Scholar]
  13. Konkel M. E, Monteville M. R, Rivera-Amill V, Joens L. 2001; The pathogenesis of Campylobacter jejuni -mediated enteritis. Curr Issues Intest Microbiol2:55–71
    [Google Scholar]
  14. Kopecko D. J, Hu L, Zaal K. J. M. 2001; Campylobacter jejuni – microtubule-dependent invasion. Trends Microbiol9:389–396[CrossRef]
    [Google Scholar]
  15. Loss R. W, Mangla J. C, Pereira M. 1980; Campylobacter colitis presenting as inflammatory bowel disease with segmental colonic ulcerations. Gastroenterology79:138–140
    [Google Scholar]
  16. Malago J. J, Koninkx J. F, Tooten P. C, van Liere E. A, van Dijk J. E. 2005; Anti-inflammatory properties of heat shock protein 70 and butyrate on Salmonella -induced interleukin-8 secretion in enterocyte-like Caco-2 cells. Clin Exp Immunol141:62–71[CrossRef]
    [Google Scholar]
  17. Masters J. R. 2002; HeLa cells 50 years on: the good, the bad and the ugly. Nat Rev Cancer2:315–319[CrossRef]
    [Google Scholar]
  18. Mellits K. H, Mullen J, Wand M, Armbruster G, Patel A, Connerton P. L, Skelly M, Connerton I. F. 2002; Activation of the transcription factor NF- κ B by Campylobacter jejuni . Microbiology148:2753–2763
    [Google Scholar]
  19. Parkhill J, Achtman M, James K. D. 25 other authors 2000; The genome sequence of the food borne pathogen Campylobacter jejuni reveals hypervariable sequences. Nature403:665–668[CrossRef]
    [Google Scholar]
  20. Price A. B, Dolby J. M, Dunscombe P. R, Stirling J. 1984; Detection of Campylobacter by immunofluorescence in stools and rectal biopsies of patients with diarrhoea. J Clin Pathol37:1007–1013[CrossRef]
    [Google Scholar]
  21. Russell R. G, Blaser M, Sarmiento J. I, Fox J. 1989; Experimental Campylobacter jejuni infection in Macaca nemestrina . Infect Immun57:1438–1444
    [Google Scholar]
  22. Russell R. G, O'Donnoghue M, DeTolla L. J, Blake D. C Jr, Zulty J. 1993; Early colonic damage and invasion of Campylobacter jejuni in experimentally challenged infant Macaca mulatta . J Infect Dis168:210–215[CrossRef]
    [Google Scholar]
  23. Sansonetti P. 2002; Host pathogen interactions: the seduction of molecular cross talk. Gut50: Suppl 3III2–III8
    [Google Scholar]
  24. Skirrow M. 1986; Campylobacter enteritis. In Medical Microbiology vol. 4 Edited by Easmon C. S. F.. New York: Academic Press;
  25. Skirrow M, Blaser M. 2000; Clinical aspects of Campylobacter infection. In Campylobacter pp. 68–88 Edited by Nachamkin I., Blaser M. J.. Washington, DC: American Society for Microbiology;
  26. Strober W. 1998; Interactions between epithelial cells and immune cells in the intestine. Ann N Y Acad Sci859:37–45[CrossRef]
    [Google Scholar]
  27. Van Spreeuwel P, Duursma G. C, Meijer C. J, Bax R, Rosekrans P. C. M, Lindeman J. 1985; Campylobacter colitis : histological immunohistochemical and ultrastructural findings. Gut26:945–951[CrossRef]
    [Google Scholar]
  28. Wassenaar T. M, Blaser M. J. 1999; Pathophysiology of Campylobacter jejuni infections of humans. Microbes Infect1:1023–1033[CrossRef]
    [Google Scholar]
  29. Weglarz L, Dzierzewicz Z, Orchel A, Szczerba J, Jaworska-kik M, Wilczok T. 2003; Biological activity of Desulfovibrio desulfuricans lipopolysaccharides evaluated via interleukin-8 secretion by Caco-2 cells. Scand J Gastroenterol38:73–79
    [Google Scholar]
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