1887

Abstract

In , an homologous gene is located in the lipo-oligosaccharide synthesis gene cluster. This study examined the effects of expression on the responsiveness of and to harsh environments. Complementation experiments showed that the gene could restore the normal morphology of the mutant, and its ability to grow without inhibition under heat, acid and osmotic stresses, but not bile stress. This indicated that the genes in and exhibit similar pleiotropic effects. Moreover, quantitative real-time RT-PCR showed that expression of the gene was upregulated under acid, heat, oxidative and osmotic stresses, but did not change under bile stress. This indicated that the gene plays a role in regulating cell responses to various environmental changes. Furthermore, deletion mutation of the gene in was lethal, indicating that the gene is essential for survival. Therefore, these results showed that expression of the gene is essential for the response of and to environmental stresses.

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2007-01-01
2019-10-18
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