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Volume 152, Issue 11

A siderophore biosynthesis gene cluster from the fish pathogen subsp. is structurally and functionally related to the high-pathogenicity island Free

Abstract

subsp. , the causative agent of fish pasteurellosis, produces a siderophore which is distinct from that produced by subsp. . Using suppression subtractive hybridization, a subsp. -specific DNA region of 35 kb was identified in strain DI21, and 11 genes were defined: , , , , , , , , , and . The sequence of the predicted proteins encoded by these genes showed significant similarity with the proteins responsible for the synthesis and transport of the siderophore yersiniabactin, encoded within the high-pathogenicity island (HPI). Southern hybridization demonstrated that this gene cluster is exclusive to some European subsp. isolates. Database searches revealed that a similar gene cluster is present in SS9 and RC385. An gene (encoding a putative non-ribosomal peptide synthetase) insertional mutant (CS31) was impaired for growth under iron-limiting conditions and unable to produce siderophores, and showed an approximately 100-fold decrease in degree of virulence for fish. The subsp. DI21 strain, but not CS31, promoted the growth of a mutant. Furthermore, a yersiniabactin-producing strain as well as purified yersiniabactin were able to cross-feed strains DI21 and CS31, suggesting that the subsp. siderophore might be functionally and structurally related to yersiniabactin. The differential occurrence among strains, and the low sequence similarity to siderophore synthesis genes described in other members of the , suggest that this genetic system might have been acquired by horizontal transfer in subsp. , and might have a common evolutionary origin with the HPI.

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  • Accepted:
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  • Published Online:
Keyword(s): A domain, adenylation domain , ArCP, aryl-carrier protein , DAHP, 3-deoxy-d-arabino-heptulosonate-7-phosphate , HPI, high-pathogenicity island , NRPS, non-ribosomal peptide synthetase and SSH, suppression subtractive hybridization
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2006-11-01
2024-04-19
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A siderophore biosynthesis gene cluster from the fish pathogen Photobacterium damselae subsp. piscicida is structurally and functionally related to the Yersinia high-pathogenicity island
Microbiology 152, 3327 (2006); https://doi.org/10.1099/mic.0.29190-0
/content/journal/micro/10.1099/mic.0.29190-0
/content/journal/micro/10.1099/mic.0.29190-0
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