1887

Abstract

The gene of the operon, which is instrumental in the control of the biosynthesis of the exopolysaccharide alginate, is a hotspot of mutation in , a micro-organism that chronically colonizes the airways of individuals with cystic fibrosis (CF). The , and genes were sequenced in nine environmental isolates from aquatic habitats, and in 37 strains isolated from 10 patients with CF, at onset or at a late stage of chronic airway colonization, in order to elucidate whether there was any association between mutation and background genotype. The 61 identified single nucleotide polymorphisms (SNPs) segregated into 18 genotypes. Acquired and stop mutations were present in 14 isolates (38 %) of five genotypes. ΔG430 was the most frequent and recurrent mutation detected in four genotypes. The classification of strains by genotype was generally concordant with that by genome-wide SpeI fragment pattern or multilocus SNP genotypes. The exceptions point to intragenic mosaicism and interclonal recombination as major forces for intraclonal evolution at the locus.

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2006-11-01
2020-08-10
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