1887

Abstract

strain A1501 isolated from rice fixes nitrogen under microaerobic conditions in the free-living state. This paper describes the properties of and mutants as well as the physical interaction between NifL and NifA proteins. A mutant strain that carried a mutation non-polar on expression retained nitrogenase activity. Complementation with a plasmid containing only led to a decrease in nitrogenase activity in both the wild-type and the mutant, suggesting that NifL acts as an antiactivator of NifA activity. Using the yeast two-hybrid system and purified protein domains of NifA and NifL, an interaction was shown between the C-terminal domain of NifL and the central domain of NifA, suggesting that NifL antiactivator activity is mediated by direct protein interaction with NifA.

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2006-12-01
2021-10-16
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