1887

Abstract

The extremely radioresistant bacterium contains two LexA homologues (LexA1 and LexA2) that are possible transcriptional regulators associated with the DNA damage response. In this study, resequencing revealed that there was an additional cytosine nucleotide (nucleotide position 612) in the gene. Purified LexA2 possessed proteolytic activity that could be stimulated by RecA. In an effort to gain an insight into the role of LexA2 in the radiation response mechanism, , and disruptant strains were generated and investigated. The intracellular level of RecA increased in and disruptant strains following -irradiation as in the wild-type strain. These results indicated that the two LexA homologues did not possess functional overlap regarding the induction of RecA. The disruptant strains exhibited a much higher resistance to -rays than the wild-type strain. Furthermore, a luciferase assay showed that promoter activation was enhanced in the disruptant strain following -irradiation. The gene encoding the novel radiation-inducible protein PprA plays a critical role in the radioresistance of . The increase in radioresistance of the disruptant strain is explained in part by the enhancement of promoter activation.

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2006-11-01
2020-10-29
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