1887

Abstract

Effects of tRNA(UGC) and its derivative devoid of the 3′-ACCA motif [tRNA(UGC)ΔACCA] on the cleavage of the ColE1-like plasmid-derived RNA I were analysed and . In an amino-acid-starved mutant, in which uncharged tRNAs occur in large amounts, three products of specific cleavage of RNA I were observed, in contrast to an otherwise isogenic host. Overexpression of tRNA(UGC), which under such conditions occurs in mostly in an uncharged form, induced RNA I cleavage and resulted in an increase in ColE1-like plasmid DNA copy number. Such effects were not observed during overexpression of the 3′-ACCA-truncated tRNA(UGC). Moreover, tRNA(UGC), but not tRNA(UGC)ΔACCA, caused RNA I cleavage in the presence of MgCl. These results strongly suggest that tRNA-dependent RNA I cleavage occurs in ColE1-like plasmid-bearing , and demonstrate that tRNA(UGC) participates in specific degradation of RNA I and . This reaction is dependent on the presence of the 3′-ACCA motif of tRNA(UGC).

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2006-12-01
2020-03-30
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