1887

Abstract

Paratuberculosis (Johne's disease) poses a significant economic problem to beef, dairy and sheep industries worldwide, and is caused by subspecies . In this study, 2D PAGE was used as a tool to investigate the virulent state of subsp. , incorporating the technique of beating the organism with zirconium/silica beads to provide a comprehensive representation of its proteome. A direct comparison of the proteomes of subsp. scraped from the terminal ileum of ovine paratuberculosis cases, and the identical strain grown , is presented. These analyses identified a set of 10 proteins whose expression is upregulated during natural infection: 1-pyrroline-5-carboxylate dehydrogenase (RocA), a putative acyl-CoA dehydrogenase (FadE14), 2-methylcitrate dehydratase (2-mcd), arginosuccinate synthase (ArgG), universal stress protein (usp), 30S ribosomal protein S2 (RpsB), peptidyl-prolyl - isomerase (PpiA), luciferase-like monooxygenase (lmo), thiosulfate sulfurtransferase (SseA) and ATP-dependent Clp protease (ClpB). Most of the proteins identified do not have obviously related functions; however, ArgG and RocA function in the same pathway, and may have a concerted action for energy production .

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2007-01-01
2020-04-10
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References

  1. Angelichio M. J., Camilli A. 2002; In vivo expression technology. Infect Immun70:6518–6523[CrossRef]
    [Google Scholar]
  2. Boon C., Dick T. 2002; Mycobacterium bovis BCG response regulator essential for hypoxic dormancy. J Bacteriol184:6760–6767[CrossRef]
    [Google Scholar]
  3. Brennan P. J., Nikaido H. 1995; The envelope of mycobacteria. Annu Rev Biochem64:29–63[CrossRef]
    [Google Scholar]
  4. Brunori L., Giannoni F., Bini L., Liberatori S., Frota C., Jenner P., Thoresen O. F., Orefici G., Fattorini L. 2004; Induction of Mycobacterium avium proteins upon infection of human macrophages. Proteomics4:3078–3083[CrossRef]
    [Google Scholar]
  5. Chiodini R. J., Merkal R. S, van Kruiningen H. J.. 1984; Ruminant paratuberculosis (Johnes disease): the current status and future prospects. Cornell Vet74:218–262
    [Google Scholar]
  6. Choy E., Whittington R. J., Marsh I., Marshall J., Campbell M. T. 1998; A method for purification and characterisation of Mycobacterium avium subsp. paratuberculosis from the intestinal mucosa of sheep with Johnes disease. Vet Microbiol64:51–60[CrossRef]
    [Google Scholar]
  7. Clarke C. J. 1997; The pathology and pathogenesis of paratuberculosis in ruminants and other species. J Comp Pathol116:217–261[CrossRef]
    [Google Scholar]
  8. Colnaghi R., Cassinelli G., Drummond M., Forlani F., Pagani S. 2001; Properties of the Escherichia coli rhodanese-like protein SseA: contribution of the active-site residue Ser240 to sulfur donor recognition. FEBS Lett500:153–156[CrossRef]
    [Google Scholar]
  9. Dozois C. M., Daigle F., Curtiss R. 2003; Identification of pathogen-specific and conserved genes expressed in vivo by an avian pathogenic Escherichia coli strain. Proc Natl Acad Sci U S A100:247–252[CrossRef]
    [Google Scholar]
  10. Frees D., Chastanet A., Qazi S., Sorensen K., Hill P., Msadek T., Ingmer H. 2004; Clp ATPases are required for stress tolerance, intracellular replication and biofilm formation in Staphylococcus aureus . Mol Microbiol54:1445–1462[CrossRef]
    [Google Scholar]
  11. Goloubinoff P., Mogk A., Peres Ben Zvi A., Tomoyasu T., Bukau B. 1999; Sequential mechanism of solubilization and refolding of stable protein aggregates by a bichaperone network. Proc Natl Acad Sci U S A96:13732–13737[CrossRef]
    [Google Scholar]
  12. Hughes V. M., Stevenson K., Sharp J. M. 2001; Improved preparation of high molecular weight DNA for pulsed-field gel electrophoresis from mycobacteria. J Microbiol Methods44:209–215[CrossRef]
    [Google Scholar]
  13. Julian R. J. 1975; A short review and some observations on Johne's disease with recommendations for control. Can Vet J16:33–43
    [Google Scholar]
  14. Kim K. I., Woo K. M., Seong I. S., Lee Z. W., Baek S. H., Chung C. H. 1998; Mutational analysis of the two ATP-binding sites in ClpB, a heat shock protein with protein activated ATPase activity in Eschericia coli . Biochem J333:671–676
    [Google Scholar]
  15. Kvint K., Nachin L., Diez A., Nystrom T. 2003; The bacterial universal stress protein: function and regulation. Curr Opin Microbiol6:140–145[CrossRef]
    [Google Scholar]
  16. LaMarca B. B. D., Zhu W., Arceneaux J. E. L., Byers B. R., Lundrigan M. D. 2004; Participation of fad and mbt genes in synthesis of mycobactin in Mycobacterium smegmatis . J Bacteriol186:374–382[CrossRef]
    [Google Scholar]
  17. Lanigan M. D., Vaughan J. A., Shiell B. J., Beddome G. J., Michalski W. P. 2004; Mycobacterial proteome extraction: comparison of disruption methods. Proteomics4:1094–1100[CrossRef]
    [Google Scholar]
  18. Mogk A., Tomoyasu T., Goloubinoff P., Rudiger S., Roder D., Langen H., Bukau B. 1999; Identification of thermolabile Escherichia coli proteins: prevention and reversion of aggregation by DnaK and ClpB. EMBO J18:6943–6949
    [Google Scholar]
  19. Morrissey J. H. 1981; Silver stain for proteins in polyacrylamide gels-a modified procedure with enhanced uniform sensitivity. Anal Biochem117:307–310[CrossRef]
    [Google Scholar]
  20. Neuhoff V., Stamm R., Eibl H. 1985; Clear background and highly sensitive protein staining with coomassie blue dyes in polyacrylamide gels-a systematic analysis. Electrophoresis6:427–448[CrossRef]
    [Google Scholar]
  21. Nystrom T., Neidhardt F. C. 1996; Effects of overproducing the universal stress protein, UspA, in Escherichia coli K-12. J Bacteriol178:927–930
    [Google Scholar]
  22. O'Toole R., Williams H. D. 2003; Universal stress proteins and Mycobacterium tuberculosis . Res Microbiol154:387–392[CrossRef]
    [Google Scholar]
  23. O'Toole R., Smeulders M. J., Blokpoel M. C., Kay E. J., Loughheed K., Williams H. D. 2003; A two-component regulator of universal stress protein expression and adaptation to oxygen starvation in Mycobacterium smegmatis . J Bacteriol185:1543–1554[CrossRef]
    [Google Scholar]
  24. Perkins D. N., Pappin D. J., Creasy D. M., Cottrell J. S. 1999; Probability-based protein identification by searching sequence databases using mass spectrometry data. Electrophoresis20:3551–3567[CrossRef]
    [Google Scholar]
  25. Rodriguez G. M., Voskuil M. I., Gold B., Schoolnik G. K., Smith I. 2002; IdeR, An essential gene in Mycobacterium tuberculosis : role of IdeR in iron-dependent gene expression, iron metabolism, and oxidative stress response. Infect Immun70:3371–3381[CrossRef]
    [Google Scholar]
  26. Squires C. L., Pedersen S. B., Ross M., Squires C. 1991; ClpB is the Escherichia coli heat shock protein F84.1. J Bacteriol173:4254–4262
    [Google Scholar]
  27. Stewart G. R., Wernisch L., Stabler R., Mangan J. A., Hinds J., Laing K. G., Young D. B., Butcher P. D. 2002; Dissection of the heat-shock response in Mycobacterium tuberculosis using mutants and microarrays. Microbiology148:3129–3137
    [Google Scholar]
  28. Timm J., Post F. A., Bekker L.-G., Walther G. B., Wainwright H. C., Manganelli R., Chan W. T., Tsenova L., Gold B.. other authors 2003; Differential expression of iron-, carbon-, and oxygen-responsive mycobacterial genes in the lungs of chronically infected mice and tuberculosis patients. Proc Natl Acad Sci U S A100:14321–14326[CrossRef]
    [Google Scholar]
  29. Stratmann J., Strommenger B., Goethe R., Dohmann K., Gerlach G. F., Stevenson K., Li L. L., Zhang Q., Kapur V., Bull T. J. 2004; A 38-kilobase pathogenicity island specific for Mycobacterium avium subsp. paratuberculosis encodes cell surface proteins expressed in the host. Infect Immun72:1265–1274[CrossRef]
    [Google Scholar]
  30. Wu G. 1998; Intestinal mucosal amino acid catabolism. J Nutr128:1249–1252
    [Google Scholar]
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