1887

Abstract

Orthologues of the serovar Typhimurium () BarA/SirA two-component system are important for biofilm formation and virulence in many -. In , SirA activates the and carbon storage regulatory RNAs and the virulence gene regulators and . The regulatory RNAs antagonize the activity of the CsrA protein, allowing translation of those same virulence genes, and inhibiting the translation of flagellar genes. In this report, it was determined that SirA and the Csr system also control the operon that encodes type 1 fimbriae. orthologues in other bacterial species, and the operon of , are known to play a role in biofilm formation; therefore, all members of the regulon were tested for biofilm production. A mutant, a double mutant, and a mutant, were all defective in biofilm formation. Conversely, inactivation of increased biofilm formation. Therefore, SirA activates , and the operon to promote biofilm formation. In turn, and promote the translation of the operon, while at the same time inhibiting the translation of flagella, which are inhibitory to biofilm formation.

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2006-11-01
2020-03-28
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