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Volume 152, Issue 10

The role of the histidine-35 residue in the cytocidal action of HM-1 killer toxin Free

Abstract

Diethylpyrocarbonate modification and site-directed mutagenesis studies of histidine-35 in HM-1 killer toxin (HM-1) have shown that a specific feature, the imidazole side chain of histidine-35, is essential for the expression of the killing activity. In subcellular localization experiments, wild-type HM-1 was in the membrane fraction of BJ1824, but not the HM-1 analogue in which histidine-35 was replaced by alanine (H35A HM-1). Neither wild-type nor H35A HM-1 was detected in cellular fractions of HM-1-resistant yeast BJ1824 Δ : :  and HM-1-insensitive yeast even after 1 h incubation. H35A HM-1 inhibited the activity of partially purified 1,3--glucan synthase from A451, and its extent was almost the same as wild-type HM-1. Co-immunoprecipitation experiments showed that wild-type and H35A HM-1 directly interact with the 1,3--glucan synthase complex. These results strongly suggest that histidine-35 has an important role in the cytocidal action of HM-1 that participates in the binding process to the HM-1 receptor protein on the cell membrane, but it is not essential for the interaction with, and inhibition of, 1,3--glucan synthase.

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Keyword(s): CD, circular dichroism , DEPC, diethylpyrocarbonate and HM-1, HM-1 killer toxin
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2006-10-01
2024-04-18
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References

  1. Antuch W, Güntert P, Wüthrich K. 1996; Ancestral β γ -crystallin precursor structure in a yeast killer toxin. Nat Struct Biol 3:662–665 [CrossRef]
     [Google Scholar]
  2. Bussey H. 1981; Physiology of killer factor in yeast. Adv Microb Physiol 22:93–122
     [Google Scholar]
  3. Bussey H, Saville D, Hutchins K, Palfree R. G. E. 1979; Binding of yeast killer toxin to a cell wall receptor on sensitive Saccharomyces cerevisiae . J Bacteriol 140:888–892
     [Google Scholar]
  4. Ellgaard L, Helenius A. 2003; Quality control in the endoplasmic reticulum. Nat Rev Mol Cell Biol 4:181–191 [CrossRef]
     [Google Scholar]
  5. Harlow E, Lane D. 1999 Using Antibodies: a Laboratory Manual Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
     [Google Scholar]
  6. Hutchens K, Bussey H. 1983; Cell wall receptor for yeast killer toxin: involvement of (1-6)- β -d-glucan. J Bacteriol 154:161–169
     [Google Scholar]
  7. Kasahara S, Inoue S. B, Mio T, Yamada T, Nakajima T, Ichishima E, Furuichi Y, Yamada H. 1994; Involvement of cell wall β -glucan in the action of HM-1 killer toxin. FEBS Lett 348:27–32 [CrossRef]
     [Google Scholar]
  8. Kashiwagi T, Kunishima N, Suzuki C, Tsuchiya F, Nikkuni S, Arata Y, Morikawa K. 1997; The novel acidophilic structure of the killer toxin from halotolerant yeast demonstrates remarkable folding similarity with a fungal killer toxin. Structure 5:81–94 [CrossRef]
     [Google Scholar]
  9. Kimura T, Kitamoto N, Matsuoka K, Nakamura K, Iimura Y, Kito Y. 1993; Isolation and nucleotide sequence of the genes encoding killer toxins from Hansenula mrakii and H. saturnus . Gene 137:265–270 [CrossRef]
     [Google Scholar]
  10. Kimura T, Kitamoto N, Kito Y, Iimura Y, Shirai T, Komiyama T, Furuichi Y, Sakka K, Ohmiya K. 1997; A novel yeast gene, RHK 1, is involved in the synthesis of the cell wall receptor for the HM-1 killer toxin that inhibits β -1,3-glucan synthesis. Mol Gen Genet 254:139–147 [CrossRef]
     [Google Scholar]
  11. Kimura T, Iimura Y, Komiyama T, Karita S, Sakka K, Ohmiya K. 1998; Killing mechanism by HM-1 killer toxin of Hansenula mrakii and application of HM-1 in fermentation process to prevent contamination with wild yeasts. Recent Res Dev Ferment Bioeng 1:225–240
     [Google Scholar]
  12. Kimura T, Kitamoto N, Ohta Y, Kito Y, Iimura Y. J. 1995; Structural relationships among killer toxins secreted from the killer strains of the genus Williopsis . J Ferment Bioeng 80:85–87 [CrossRef]
     [Google Scholar]
  13. Kimura T, Komiyama T, Furuichi Y, Iimura Y, Karita S, Sakka K, Ohmiya K. 1999; N -glycosylation is involved in the sensitivity of Saccharomyces cerevisiae to HM-1 killer toxin secreted from Hansenula mrakii IFO 0895. Appl Microbiol Biotechnol 51:176–184 [CrossRef]
     [Google Scholar]
  14. Kishida M, Tokunaga M, Katayose Y, Yajima H, Kawamura-Watabe A, Hishinuma F. 1996; Isolation and genetic characterization of pGKL killer-insensitive mutants (iki) from Saccharomyces cerevisiae . Biosci Biotechnol Biochem 60:798–801 [CrossRef]
     [Google Scholar]
  15. Komiyama T, Ohta T, Urakami H, Shiratori Y, Takasuka T, Sato M, Watanabe T, Furuichi Y. 1996; Pore formation on proliferating yeast Saccharomyces cerevisiae cell buds by HM-1 killer toxin. J Biochem 119:731–736 [CrossRef]
     [Google Scholar]
  16. Komiyama T, Kimura T, Furuichi Y. 2002; Round shape enlargement of the yeast spheroplast of Saccharomyces cerevisiae by HM-1 toxin. Biol Pharm Bull 25:959–965 [CrossRef]
     [Google Scholar]
  17. Komiyama T, Zhang Q, Miyamoto M, Selvakumar D, Furuichi Y. 2004; Monoclonal antibodies and sandwich ELISA for quantitation of HM-1 killer toxin. Biol Pharm Bull 27:691–693 [CrossRef]
     [Google Scholar]
  18. Kowalski J. M, Parekh R. N, Mao J, Wittrup K. D. 1998; Protein folding stability can determine the efficiency of escape from endoplasmic reticulum quality control. J Biol Chem 273:19453–19458 [CrossRef]
     [Google Scholar]
  19. Kunishima N, Kashigawa T, Suzuki C, Tsuchiya F, Arata Y, Morikawa K. 1997; Crystallization and preliminary X-ray diffraction studies of a novel killer toxin from a halotolerant yeast Pichia farinosa . Acta Crystallogr D Biol Crystallogr 53:112–113 [CrossRef]
     [Google Scholar]
  20. Kurzweilova H, Sigler K. 1994; Kinetic studies of killer toxin K1 binding to yeast cells indicate two receptor populations. Arch Microbiol 162:211–214 [CrossRef]
     [Google Scholar]
  21. Marquina D, Santos A, Peinado J. M. 2002; Biology of killer yeasts. Int Microbiol 5:65–71 [CrossRef]
     [Google Scholar]
  22. Martinac B, Zhu H, Kubalski A, Zhou X. 1990; Yeast K1 killer toxin forms ion channels in sensitive yeast spheroplasts and in artificial liposomes. Proc Natl Acad Sci U S A 87:6228–6232 [CrossRef]
     [Google Scholar]
  23. Miles E. W. 1977; Modification of histidyl residues in proteins by diethylpyrocarbonate. Methods Enzymol 47:431–442
     [Google Scholar]
  24. Miyamoto M, Han G.-D, Kimura T, Furuichi Y, Komiyama T. 2005; Alanine-scanning mutagenesis of HM-1 killer toxin and the essential residues for killing activity. J Biochem 137:517–522 [CrossRef]
     [Google Scholar]
  25. Sambrook J, Fritsh E. F, Maniatis T. 1989 Molecular Cloning: a Laboratory Manual, 2nd edn.. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
     [Google Scholar]
  26. Schmitt M. J, Compain P. 1995; Killer-toxin-resistant kre12 mutants of Saccharomyces cerevisiae : genetic and biochemical evidence for a secondary K1 membrane receptor. Arch Microbiol 164:435–443 [CrossRef]
     [Google Scholar]
  27. Schäger H, von Jagow G. 1987; Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal Biochem 166:368–379 [CrossRef]
     [Google Scholar]
  28. Shirai T, Kimura T, Furuichi Y, Komiyama T. 2000; The involvement of two specific arginine residues in the action of HM-1 killer toxin was deduced from site-directed mutagenesis. Biol Pharm Bull 23:998–1000 [CrossRef]
     [Google Scholar]
  29. Suzuki C, Ando Y, Machida S. 2001; Interaction of SMKT, a killer toxin produced by Pichia farinosa , with the yeast cell membranes. Yeast 18:1471–1478 [CrossRef]
     [Google Scholar]
  30. Takasuka T, Komiyama T, Furuichi Y, Watanabe T. 1995; Cell wall synthesis specific cytocidal effect of Hansenula mrakii toxin-1 on Saccharomyces cerevisiae . Cell Mol Biol Res 41:575–581
     [Google Scholar]
  31. Tipper D. J, Schmitt M. J. 1991; Yeast dsRNA viruses: replication and killer phenotypes. Mol Microbiol 5:2331–2338 [CrossRef]
     [Google Scholar]
  32. Tsang V. C, Peralta J. M, Simons A. R. 1983; Enzyme-linked immunoelectrotransfer blot techniques (EITB) for studying the specificities of antigens and antibodies separated by gel electrophoresis. Methods Enzymol 92:377–391
     [Google Scholar]
  33. Yamamoto T, Imai M, Tachibana K, Mayumi M. 1986; Application of monoclonal antibodies to the isolation and characterization of a killer toxin secreted by Hansenula mrakii . FEBS Lett 195:253–257 [CrossRef]
     [Google Scholar]
  34. Yamamoto T, Uchida K, Hiratani T, Miyazaki T, Yagiu J, Yamaguchi H. 1988; In vitro activity of the killer toxin from yeast Hansenula mrakii against yeasts and molds. J Antibiot 41:398–403 [CrossRef]
     [Google Scholar]
  35. Young T. W, Yagiu M. 1978; A comparison of the killer character in different yeasts and its classification. Antonie van Leeuwenhoek 44:59–77 [CrossRef]
     [Google Scholar]
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The role of the histidine-35 residue in the cytocidal action of HM-1 killer toxin
Microbiology 152, 2951 (2006); https://doi.org/10.1099/mic.0.29100-0
/content/journal/micro/10.1099/mic.0.29100-0
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