1887

Abstract

Vfr, a global regulator of virulence factors, is a homologue of the cAMP receptor protein, CRP. Vfr is 91 % similar to CRP and maintains many residues important for CRP to bind cAMP, bind DNA, and interact with RNA polymerase at target promoters. While can complement an mutant in -galactosidase production, tryptophanase production and catabolite repression, can only complement a subset of Vfr-dependent phenotypes in . Using specific CRP binding site mutations, it is shown that Vfr requires the same nucleotides as CRP for optimal transcriptional activity from the promoter. In contrast, CRP did not bind Vfr target sequences in the promoters of the and genes. Footprinting analysis revealed Vfr protected sequences upstream of , , and the quorum sensing regulator , that are similar to but significantly divergent from the CRP consensus binding sequence, and Vfr causes similar DNA bending to CRP in bound target sequences. Using a preliminary Vfr consensus binding sequence deduced from the Vfr-protected sites, Vfr target sequences were identified upstream of the virulence-associated genes , , , and , and in the , , intergenic regions. From these sequences the Vfr consensus binding sequence, 5′-ANWWTGNGAWNY : AGWTCACAT-3′, was formulated. This study suggests that Vfr shares many of the same functions as CRP, but has specialized functions, at least in terms of DNA target sequence binding, required for regulation of a subset of genes in its regulon.

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2006-12-01
2019-11-22
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