1887

Abstract

regulates gene expression in response to environmental conditions, including temperature, pH, redox potential and host factors. encodes a PerR homologue designated BosR, which presumably serves as a global regulator of genes involved in the oxidative stress response. Infectious strain B31 is resistant to oxidative stressors , whereas the non-infectious isolate was sensitive due, in part, to a point mutation that converts an arginine to a lysine at residue 39 of BosR. Subsequent insertional inactivation of this allele ( : : kan) restored resistance to oxidative stressors. These observations suggest that the non-infectious  : : kan strain may transcribe genes that are also expressed in infectious cells, but are repressed in the background, thus explaining the different oxidative stress phenotypes observed between these isolates. To test this hypothesis, macroarray technology and quantitative RT-PCR were utilized to compare the transcriptional profiles from the isogenic and  : : kan isolates. Array data indicated that 88 ORFs were significantly expressed in the absence of BosRR39K. Since most affected genes mapped to the chromosome, it is likely that these genes define an important physiologic response for . Included within the genes identified was the detoxification gene , as well as other loci not overtly linked to oxidative stress. These results suggest that a putative BosR regulon, as defined by the allele, is required to combat toxic oxidative intermediates, but may also be involved in adaptive strategies that are independent of reactive oxygen species.

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2006-09-01
2019-10-17
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