1887

Abstract

The potential functions have been investigated of two proteins in R1 predicted to be involved in the maintenance and integrity of the S layer: the hexagonally packed intermediate (Hpi) protein, and SlpA (DR2577), a homologue of an S-layer SlpA protein in . Deletion of the gene had little effect on the structure of the cell envelope or on shear- or solvent-induced stress responses. However, deletion of the gene caused substantial alterations in cell envelope structure, and a significant defect in resistance to solvent and shear stresses compared to the wild-type. Ultrastructural analysis of mutant cells indicated loss of much of the outer Hpi protein carbohydrate coat, the ‘pink envelope’, and the membrane-like backing layer. Together these results suggest that the SlpA protein may be involved in attachment of the Hpi surface layer to the inner cell envelope, and that SlpA may play an important role in the maintenance of cell envelope integrity in .

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2006-09-01
2020-09-28
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References

  1. Baumeister W, Kübler O. 1978; Topographic study of the cell surface of Micrococcus radiodurans . Proc Natl Acad Sci U S A75:5525–5528[CrossRef]
    [Google Scholar]
  2. Baumeister W, Zingsheim H. P, Kübler O. 1981; The structure of the cell envelope of Micrococcus radiodurans as revealed by metal shadowing and decoration. J Ultrastruct Res75:60–71[CrossRef]
    [Google Scholar]
  3. Baumeister W, Karrenberg F, Rachel R, Engel A, Ten Heggeler B, Saxton W. O. 1982; The major cell envelope protein of Micrococcus radiodurans (R1). Structural and chemical characterization. Eur J Biochem125:535–544[CrossRef]
    [Google Scholar]
  4. Baumeister W, Barth M, Hegerl R, Guckenberger R, Hahn M, Saxton W. O. 1986; Three-dimensional structure of the regular surface layer (HPI layer) of Deinococcus radiodurans . J Mol Biol187:241–250[CrossRef]
    [Google Scholar]
  5. Beveridge T. J, Pouwels P. H, Sára M. 22 other authors 1997; Functions of S-layers. FEMS Microbiol Rev20:99–149[CrossRef]
    [Google Scholar]
  6. Couture-Tosi E, Delacroix H, Mignot T, Mesnage S, Chami M, Fouet A, Mosser G. 2002; Structural analysis and evidence for dynamic emergence of Bacillus anthracis S-layer networks. J Bacteriol184:6448–6456[CrossRef]
    [Google Scholar]
  7. Emde B, Wehrli E, Baumeister W. 1980; The topography of the cell wall of Micrococcus radiodurans . Electron Microsc2:460–461
    [Google Scholar]
  8. Gatlin C. L, Kleemann G. R, Hays L. G, Link A. J, Yates J. R 3rd. 1998; Protein identification at the low femtomole level from silver-stained gels using a new fritless electrospray interface for liquid chromatography-microspray and nanospray mass spectrometry. Anal Biochem263:93–101[CrossRef]
    [Google Scholar]
  9. Gentner N. E, Mitchel R. E. 1975; Ionizing radiation-induced release of a cell surface nuclease from Micrococcus radiodurans . Radiat Res61:204–215[CrossRef]
    [Google Scholar]
  10. Kübler O., Baumeister W. 1978; The structure of a periodic cell wall component (HPI-layer of Micrococcus radiodurans ). Cytobiologie17:1–9
    [Google Scholar]
  11. Kuen B, Lubitz W. 1996; Analysis of S-layer proteins and genes. Chapter 4 in Crystalline Bacterial Cell Surface Proteins pp 77–102 Edited by Sleytr P. M. U. B., Pum D., Sára M.. Austin, TX: R. G. Landes;
    [Google Scholar]
  12. Kuen B, Lubitz W, Sára M. 1996; Heterologous expression and self-assembly of the S-layer protein SbsA of Bacillus stearothermophilus in Escherichia coli . Mol Microbiol19:495–503[CrossRef]
    [Google Scholar]
  13. Marx C. J, Lidstrom M. E. 2002; Broad-host-range cre-lox system for antibiotic marker recycling in Gram-negative bacteria. Biotechniques33:1062–1067
    [Google Scholar]
  14. Meima R, Lidstrom M. E. 2000; Characterization of the minimal replicon of a cryptic Deinococcus radiodurans SARK plasmid and development of versatile Escherichia coli - D. radiodurans shuttle vectors. Appl Environ Microbiol66:3856–3867[CrossRef]
    [Google Scholar]
  15. Murray R. G. E. 1992; The deinococci. In The Prokaryotes: a Handbook on the Biology of Bacteria: Ecophysiology, Isolation, Identification, Applications Edited by Ballows H. G. T. A., Hader M. Dworkin W., Schleifer K.. New York: Springer;
    [Google Scholar]
  16. Olabarria G, Carrascosa J. L, Berenguer J, de Pedro M. A. 1996; A conserved motif in S-layer proteins is involved in peptidoglycan binding in Thermus thermophilus . J Bacteriol178:4765–4772
    [Google Scholar]
  17. Peters J, Baumeister W. 1986; Molecular cloning, expression, and characterization of the gene for the surface (HPI)-layer protein of Deinococcus radiodurans in Escherichia coli . J Bacteriol167:1048–1054
    [Google Scholar]
  18. Peters J, Peters M, Lottspeich F, Baumeister W, Schäfer W. 1987; Nucleotide sequence analysis of the gene encoding the Deinococcus radiodurans surface protein, derived amino acid sequence, and complementary protein chemical studies. J Bacteriol169:5216–5223
    [Google Scholar]
  19. Rachel R, Pum D, Komrska J, Rieger G, Stetter K. O, Šmarda J, Šmajs D, Krzyzánek V. 1997; Fine structure of S-layers. FEMS Microbiol Rev20:13–23[CrossRef]
    [Google Scholar]
  20. Sambrook J, Fritsch E. F, Maniatis T. 1989; Molecular Cloning: a Laboratory Manual, 2nd edn.. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  21. Schmid A. K, Howell H. A, Battista J. R, Peterson S. N, Lidstrom M. E. 2005; Global transcriptional and proteomic analysis of the Sig1 heat shock regulon of Deinococcus radiodurans . J Bacteriol187:3339–3351[CrossRef]
    [Google Scholar]
  22. Shevchenko A, Wilm M, Vorm O, Mann M. 1996; Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels. Anal Chem68:850–858[CrossRef]
    [Google Scholar]
  23. Sleytr U. B, Messner P, Pum D, Sára M. 1993; Crystalline bacterial cell surface layers. Mol Microbiol10:911–916[CrossRef]
    [Google Scholar]
  24. Thompson B. G, Murray R. G. E, Boyce J. F. 1982; The association of the surface array and the outer membrane of Deinococcus radiodurans . Can J Microbiol20:1081–1088
    [Google Scholar]
  25. Thornley M. J, Horne R. W, Glauert A. M. 1965; The fine structure of Micrococcus radiodurans . Arch Mikrobiol51:267–289[CrossRef]
    [Google Scholar]
  26. Udupa K. S, O'Cain P. A, Mattimore V, Battista J. R. 1994; Novel ionizing radiation-sensitive mutants of Deinococcus radiodurans . J Bacteriol176:7439–7446
    [Google Scholar]
  27. White O, Eisen J. A, Heidelberg J. F.29 other authors 1999; Genome sequence of the radioresistant bacterium Deinococcus radiodurans R1. Science286:1571–1577[CrossRef]
    [Google Scholar]
  28. Work E, Griffiths H. 1968; Morphology and chemistry of cell walls of Micrococcus radiodurans . J Bacteriol95:641–657
    [Google Scholar]
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