1887

Abstract

Adenylyltransferase, GlnE, has a predicted role in controlling the enzymic activity of glutamine synthetase, the key enzyme in ammonia assimilation. It was previously demonstrated that is an essential gene in . is located downstream of , one of four glutamine synthetases. The expression of GlnE under various conditions was determined. Although a co-transcript of and was detectable, the major transcript was monocistronic. A transcriptional start site immediately upstream of was identified and it was shown by site-directed mutagenesis that the predicted −10 region is a functional promoter. It was demonstrated that in a background P was up-regulated in ammonia- or glutamine-containing media.

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2006-09-01
2020-04-07
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