1887

Abstract

The (-like) genes encode putative transcription factors unique to actinomycetes. This study characterized the promoter element of one of the seven genes of , (Rv3219c). The results reveal that is transcribed by a class I-type cAMP receptor protein (CRP)-dependent promoter, harbouring a CRP-binding site positioned at −58.5 with respect to its transcription start point. promoter activity analysis and electrophoretic mobility shift assays suggest that the expression of is indeed regulated by cAMP-dependent binding of CRP (encoded by the gene Rv3676) to the 5′ untranslated region (5′UTR). -Galactosidase gene fusion analysis revealed induction of the promoter in on addition of exogenous dibutyric cAMP (a diffusible cAMP analogue) only when an intact CRP-binding site was present. These results indicate that transcription is regulated in part by cAMP levels via direct binding of cAMP-activated CRP to a consensus CRP-binding site in the 5′UTR.

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2006-09-01
2020-01-23
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