RT Journal Article SR Electronic(1) A1 Cho, Byung-Kwan A1 Knight, Eric M. A1 Palsson, Bernhard Ø.YR 2006 T1 Transcriptional regulation of the fad regulon genes of Escherichia coli by ArcA JF Microbiology, VO 152 IS 8 SP 2207 OP 2219 DO https://doi.org/10.1099/mic.0.28912-0 PB Microbiology Society, SN 1465-2080, AB ArcA is a global transcription factor required for optimal growth of Escherichia coli during anaerobic growth. In this study, the role of ArcA on the transcriptional regulatory subnetwork of the fad regulon was investigated. Gene expression profiles of deletion mutants (ΔarcA, ΔfadR and ΔarcA/ΔfadR) indicated that (i) ArcA is a major transcription factor for the transcriptional regulation of fatty acid metabolism in the absence of oxygen, and (ii) ArcA and FadR cooperatively regulate the fad regulon under anaerobic conditions. To determine the direct interaction between ArcA and the promoters of the fad regulon genes, chromatin immunoprecipitation (ChIP) analysis was performed. ChIP analysis suggested that ArcA directly binds to the promoter regions of the fad regulon genes in vivo. An ArcA-binding motif was identified from known binding sequences and predicted putative binding sites in the promoter regions of the fad regulon genes. These results indicate that ArcA directly represses the expression of fad regulon genes during anaerobic growth., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.28912-0