1887

Abstract

Sulphate- or sulphur-reducing bacteria with known or draft genome sequences (, G20, [draft], and ) all contain or gene clusters encoding succinate : quinone oxidoreductases. or genes are missing. The presence and function of succinate dehydrogenase versus fumarate reductase was studied. (strain Essex 6) grew by fumarate respiration or by fumarate disproportionation, and contained fumarate reductase activity. lacked fumarate respiration and contained succinate dehydrogenase activity. Succinate oxidation by the menaquinone analogue 2,3-dimethyl-1,4-naphthoquinone depended on a proton potential, and the activity was lost after degradation of the proton potential. The membrane anchor SdhC contains four conserved His residues which are known as the ligands for two haem B residues. The properties are very similar to succinate dehydrogenase of the Gram-positive (menaquinone-containing) , which uses a reverse redox loop mechanism in succinate : menaquinone reduction. It is concluded that succinate dehydrogenases from menaquinone-containing bacteria generally require a proton potential to drive the endergonic succinate oxidation. Sequence comparison shows that the SdhC subunit of this type lacks a Glu residue in transmembrane helix IV, which is part of the uncoupling E-pathway in most non-electrogenic FrdABC enzymes.

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2006-08-01
2020-04-10
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