1887

Abstract

The reduction of 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide inner salt (XTT) and other tetrazolium salts is widely used as an assay for bacterial, fungal and mammalian cell viability, but the genes encoding the reductase activities have not been defined. Here, it was shown that XTT and plasma membrane ferric reductase activities were 10–40-fold greater in than in . XTT reductase activity was induced fivefold in grown in low-iron conditions compared with iron-replete conditions, and for cells grown in unbuffered (pH 4.0–4.4) medium, XTT reductase activity was largely dependent on . XTT reductase activity of grown in medium buffered to pH 6.8 was independent of but, nonetheless, was upregulated in cells deprived of iron. Reduction of 2-(4,5-dimethyl-2-thiazolyl)-3,5-diphenyl-2H-tetrazolium bromide (MTT), a membrane-permeable tetrazolium salt, occurred at an intracellular location and was independent of . However, MTT activity was induced by iron deprivation in but not in . possessed multiple iron- and pH-regulated reductase activities capable of reducing tetrazolium salts, but, when grown in unbuffered medium, was required for XTT reductase activity.

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2006-08-01
2019-11-13
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