@article{mbs:/content/journal/micro/10.1099/mic.0.28820-0, author = "Pham, Vinh D. and Shebelut, Conrad W. and Jose, Ivy R. and Hodgson, David A. and Whitworth, David E. and Singer, Mitchell", title = "The response regulator PhoP4 is required for late developmental events in Myxococcus xanthus", journal= "Microbiology", year = "2006", volume = "152", number = "6", pages = "1609-1620", doi = "https://doi.org/10.1099/mic.0.28820-0", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.28820-0", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", keywords = "QPCR, quantitative PCR", keywords = "MI, magnesium-independent", keywords = "TCS, two-component system", abstract = "Phosphate regulation is complex in the developmental prokaryote Myxococcus xanthus, and requires at least four two-component systems (TCSs). Here, the identification and characterization of a member of one TCS, designated PhoP4, is reported. phoP4 insertion and in-frame deletion strains caused spore viability to be decreased by nearly two orders of magnitude, and reduced all three development-specific phosphatase activities by 80–90 % under phosphate-limiting conditions. Microarray and quantitative PCR analyses demonstrated that PhoP4 is also required for appropriate expression of the predicted pstSCAB–phoU operon of inorganic phosphate assimilation genes. Unlike the case for the other three M. xanthus Pho TCSs, the chromosomal region around phoP4 does not contain a partner histidine kinase gene. Yeast two-hybrid analyses reveal that PhoP4 interacts reciprocally with PhoR2, the histidine kinase of the Pho2 TCS; however, the existence of certain phenotypic differences between phoP4 and phoR2 mutants suggests that PhoP4 interacts with another, as-yet unidentified, histidine kinase.", }