1887

Abstract

Phosphate regulation is complex in the developmental prokaryote , and requires at least four two-component systems (TCSs). Here, the identification and characterization of a member of one TCS, designated PhoP4, is reported. insertion and in-frame deletion strains caused spore viability to be decreased by nearly two orders of magnitude, and reduced all three development-specific phosphatase activities by 80–90 % under phosphate-limiting conditions. Microarray and quantitative PCR analyses demonstrated that PhoP4 is also required for appropriate expression of the predicted operon of inorganic phosphate assimilation genes. Unlike the case for the other three Pho TCSs, the chromosomal region around does not contain a partner histidine kinase gene. Yeast two-hybrid analyses reveal that PhoP4 interacts reciprocally with PhoR2, the histidine kinase of the Pho2 TCS; however, the existence of certain phenotypic differences between and mutants suggests that PhoP4 interacts with another, as-yet unidentified, histidine kinase.

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2006-06-01
2019-11-13
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