1887

Abstract

Polyunsaturated fatty acids (PUFAs), including linoleic acid (C18 : 2) and -linolenic acid (C18 : 3), are major components of membranes. PUFAs are produced from monounsaturated fatty acids by several fatty acid desaturases (FADs) in many fungi, but , and humans do not have these enzymes. Although the fungal pathogen produces C18 : 2 and C18 : 3, the enzymes that synthesize them have not yet been investigated. In this report, two ORFs, and , were identified based on their homology to other yeast FADs, and and gene disruptants were constructed. Δ and Δ lost their ability to produce C18 : 2 and C18 : 3, respectively. Furthermore, cells expressing Fad2p converted palmitoleic acid (C16 : 1) and C18 : 1 to hexadecadienoic acid (C16 : 2) and C18 : 2, respectively, and Fad3p-expressing cells converted C18 : 2 to C18 : 3. These results strongly supported that encodes the Δ12 FAD and that encodes the 3 FAD. However, phenotypic analysis demonstrated that the presence of these PUFAs did not affect the virulence to mice, or morphogenesis in the culture media used to induce morphological change of .

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2006-05-01
2021-05-13
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