1887

Abstract

Two kinds of nucleoside hydrolases (NHs) encoded by and were cloned from using - and -defective . Sequence analysis revealed that NH 1 was a protein of 337 aa with a deduced molecular mass of 35 892 Da, whereas NH 2 consisted of 308 aa with a calculated molecular mass of 32 310 Da. Experiments with crude extracts of IPTG-induced CGSC 6885(pTNU23) and 6885(pTNI12) indicated that the Rih1 enzyme could catalyse the hydrolysis of uridine and cytidine and showed pyrimidine-specific ribonucleoside hydrolase activity. Rih2 was able to hydrolyse both purine and pyrimidine ribonucleosides with the following order of activity – inosine>adenosine>uridine>guanosine>xanthosine>cytidine – and was classified in the non-specific NHs family. and deletion mutants displayed a decrease in cell growth on minimal medium supplemented with pyrimidine and purine/pyrimidine nucleosides, respectively, compared with the wild-type strain. Growth of each mutant was substantially complemented by introducing and , respectively. Furthermore, disruption of both and led to the inability of the mutant to utilize purine and pyrimidine nucleosides as sole carbon source on minimal medium. These results indicated that and play major roles in the salvage pathways of nucleosides in this micro-organism.

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2006-04-01
2020-08-06
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vol. , part 4, pp. 1169-1177

There is an error in the published version of Fig. 3 in this paper. The gene name appears twice and should only appear in one place after 'Ribonucleoside hydrolase 1'. The correct version of the figure is presented below.



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