1887

Abstract

The presence of a multicopy chromosome, with each copy containing two rRNA operons ( and ), has been an obstacle to analysing mutated rRNA in PCC 7942. To create a system for expressing homogeneous mutated rRNA, the chromosomal operons were sequentially inactivated and a final strain was successfully obtained with all the chromosomal operons inactivated but carrying a replaceable multicopy plasmid containing a single operon. The lag time required for growth response on dark/light shift of mutant strains with chromosomal or inactivated was increased 50 % over that of the wild-type strain; however, the presence of the plasmid-borne operon restored the lag time. The doubling time of mutant strains carrying only a functional operon, but not strains carrying only a functional operon, was significantly longer than that of the wild-type strain. A strain in which essentially all the cellular 23S rRNA contained the mutation C2588A was temperature sensitive at 16 °C and 45 °C. Position C2588 is equivalent to C2611 of the peptidyltransferase centre in domain V of 23S rRNA.

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2006-05-01
2021-03-05
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