1887

Abstract

An operon encoding a member of the family of ATP-binding cassette (ABC) divalent metal ion transporters, homologous to SitABCD, has been identified in the avian pathogenic (APEC) strain 7122. The genes were located on the virulence plasmid pAPEC-1, and were highly similar at the nucleotide level to the chromosomally encoded genes present in spp. A cloned copy of conferred increased growth upon a siderophore-deficient strain grown in nutrient broth supplemented with the chelator 2,2′-dipyridyl. Ion rescue demonstrated that Sit-mediated growth promotion of this strain was due to the transport of iron. SitABCD mediated increased transport of both iron and manganese as demonstrated by uptake of Fe, Fe or Mn in K-12 strains deficient for the transport of iron () and manganese () respectively. Isotope uptake and transport inhibition studies showed that in the iron transport deficient strain, SitABCD demonstrated a greater affinity for iron than for manganese, and SitABCD-mediated transport was higher for ferrous iron, whereas in the manganese transport deficient strain, SitABCD demonstrated greater affinity for manganese than for iron. Introduction of the APEC genes into an K-12 mutant also conferred increased resistance to the bactericidal effects of hydrogen peroxide. APEC strain 7122 derivatives lacking either a functional SitABCD or a functional MntH transport system were as resistant to hydrogen peroxide as the wild-type strain, whereas a Δ Δ double mutant was more sensitive to hydrogen peroxide. Overall, the results demonstrate that in SitABCD represents a manganese and iron transporter that, in combination with other ion transport systems, may contribute to acquisition of iron and manganese, and resistance to oxidative stress.

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2006-03-01
2019-11-20
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