1887

Abstract

RC-14 has previously been shown to inhibit infection in a rat surgical-implant model. To investigate the basis for this, communication events between the two bacterial species were examined. RC-14 and Newman were grown in a co-culture apparatus that physically separates the two species, while allowing the passage of soluble compounds. Using two-dimensional gel electrophoresis (2D-E), protein expression changes in were analysed in response to co-culture with medium alone, RC-14, and a strain that did not inhibit infection in the rat model. It was observed that one protein in particular, identified as staphylococcal superantigen-like protein 11 (SSL11), showed a dramatic decrease in expression in response to growth with RC-14. Genetic reporters that placed both and under the transcriptional control of the SSL11 promoter confirmed the 2D-E results. Interestingly, using similar reporter gene experiments, it was observed that the P3 promoter from the staphylococcal accessory gene regulator () locus also showed a decrease in expression in response to growth in the presence of RC-14. It was further demonstrated that RC-14 supernatant contained small unidentified molecules that were able to repress the SSL11 and P3 promoters, but the repression of SSL11 occurred independently of the system. These results suggest that RC-14 has the potential to alter the virulence of via secretion of cell–cell signalling molecules.

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2006-04-01
2019-10-18
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