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Volume 152, Issue 4

The sulfonated osmolyte -methyltaurine is dissimilated by and by with release of methylamine Free

Abstract

Selective enrichments yielded bacterial cultures able to utilize the osmolyte -methyltaurine as sole source of carbon and energy or as sole source of fixed nitrogen for aerobic growth. Strain MT1, which degraded -methyltaurine as a sole source of carbon concomitantly with growth, was identified as a strain of . Stoichiometric amounts of methylamine, whose identity was confirmed by matrix-assisted, laser-desorption ionization time-of-flight mass spectrometry, and of sulfate were released during growth. Inducible -methyltaurine dehydrogenase, sulfoacetaldehyde acetyltransferase (Xsc) and a sulfite dehydrogenase could be detected. Taurine dehydrogenase was also present and it was hypothesized that taurine dehydrogenase has a substrate range that includes -methyltaurine. Partial sequences of a -like gene (encoding the putative large component of taurine dehydrogenase) and an gene were obtained by PCR with degenerate primers. Strain N-MT utilized -methyltaurine as a sole source of fixed nitrogen for growth and could also utilize the compound as sole source of carbon. This bacterium was identified as a strain of . This organism also expressed inducible (-methyl)taurine dehydrogenase, Xsc and a sulfite dehydrogenase. The presence of a gene cluster with high identity to a larger cluster from NKNCYSA, which is now known to dissimilate -methyltaurine via Xsc, allowed most of the overall pathway, including transport and excretion, to be defined. -Methyltaurine is thus another compound whose catabolism is channelled directly through sulfoacetaldehyde.

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  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): DCPIP, dichlorophenol indophenol and MALDI-TOF-MS, matrix-assisted laser-desorption ionization time-of-flight mass spectrometry
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2006-04-01
2024-04-23
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The sulfonated osmolyte N-methyltaurine is dissimilated by Alcaligenes faecalis and by Paracoccus versutus with release of methylamine
Microbiology 152, 1179 (2006); https://doi.org/10.1099/mic.0.28622-0
/content/journal/micro/10.1099/mic.0.28622-0
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