1887

Abstract

In the plant-pathogenic mollicute , spiralin is the major lipoprotein at the cell surface and is thought to be one of the components involved in the interactions of the spiroplasma with its insect vector. With the aim of identifying surface proteins other than spiralin, monoclonal antibodies (mAbs) were produced by immunization of mice with the spiralin-defective mutant GII3-9a2. mAb 10G3 was found to react with several polypeptides of 43–47 and 80–95 kDa, all of which were detected in the detergent phase after Triton X-114 partitioning of proteins. Mass spectrometry (MALDI-TOF) analyses of the two major polypeptides P47 and P80 of GII3-9a2, reacting with mAb 10G3, revealed that P47 was a processed product and represented the C-terminal moiety of P80. Search for sequence homologies revealed that P80 shared strong similarities with the adhesion-related protein P89 (Sarp1) of BR3, and is one (named Scarp4a) of the eight Scarps encoded by the GII-3 genome. The eight genes are carried by plasmids pSci1–5. Western immunoblotting of proteins with mAb 10G3 revealed that, in contrast to the insect-transmissible strain GII-3, the non-insect-transmissible strains ASP-1, R8A2 and 44 did not express Scarps. Southern blot hybridization experiments indicated that these strains possessed no genes, and did not carry plasmids pSci1–5. However, strain GII3-5, lacking pSci5, was still efficiently transmitted, showing that, in the genetic background of GII-3, the pSci5-encoded genes, and in particular , and , are not essential for insect transmission. Whether plasmid-encoded genes are involved in transmission of by its leafhopper vector remains to be determined.

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2006-03-01
2020-09-20
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