1887

Abstract

The role of cytochrome , encoded by , and cytochrome , encoded by , in electron transfer to the nitrite reductase of 2.4.3 was investigated using both and approaches. Both and were isolated, sequenced and insertionally inactivated in strain 2.4.3. Deletion of either gene alone had no apparent effect on the ability of to reduce nitrite. In a double mutant, nitrite reduction was largely inhibited. However, the expression of the nitrite reductase gene from a heterologous promoter substantially restored nitrite reductase activity in the double mutant. Using purified protein, a turnover number of 5 s was observed for the oxidation of cytochrome by nitrite reductase. In contrast, oxidation of only resulted in a turnover of ∼0·1 s. The turnover experiments indicate that is a major electron donor to nitrite reductase but is probably not. Taken together, these results suggest that there is likely an unidentified electron donor, in addition to , that transfers electrons to nitrite reductase, and that the decreased nitrite reductase activity observed in the double mutant probably results from a change in expression.

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2006-05-01
2024-03-28
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