1887

Abstract

Group B streptococci (GBS) are pathogens of both neonates and adults, with serotype III strains in particular being associated with invasive disease and meningitis. In this study, a novel GBS surface antigen, , was found to be co-expressed with the previously reported antigen on an identical subset of serotype III GBS. Expression of / on the surface of serotype III GBS was shown to distinguish the restriction digest pattern (RDP) III-3 and multilocus sequence typing (ST)-17 lineage. -Specific antibodies were reactive with a unique, high-molecular-mass, serine-rich repeat protein (Srr-2) found exclusively in RDP III-3 strains. The gene encoding Srr-2 was located within a putative accessory secretory locus that included and homologues and had a genetic organization similar to that of the / locus of staphylococci. In contrast, serotype III /-negative strains and strains representative of serotypes Ia, Ib, Ic and II shared a common Srr-encoding gene, , and an organization of the / locus similar to that of previously reported serotype Ic, /-negative serotype III and serotype V GBS strains. Representative serotype III /-positive strains had LD values 3–4 logs less than those of serotype III /-negative strains in a neonatal mouse model of infection. These results indicate that the RDP III-3/ST-17 lineage expresses Srr-2 and is highly virulent in an model of neonatal sepsis.

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2006-04-01
2020-04-02
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