1887

Abstract

The reason(s) for glucose sensitivity in certain cyanobacterial strains is poorly understood. Inactivation of genes encoding the putative sensor kinase Hik31 in sp. strain PCC 6803 resulted in a mutant unable to grow in the presence of -glucose. Sensitivities to -glucose, its analogue 2-deoxy--glucose, and fructose, were alleviated in mutants in which , encoding the glucose transporter, was inactivated. These data indicate that permeation of these substrates is required to inflict cell death. The mutant Δ, and the glucose-sensitive strain of , do not possess glucokinase activity, although a transcript originating from , encoding glucokinase, is present. Inactivation of led to severe sensitivity to glucose, indicating that the presence of glucose itself, within the cells, inflicted this sensitivity. On the other hand, sensitivity to 2-deoxy--glucose was lower in Δ, thus distinguishing between the effect of glucose itself and that of its analogue, which, in the absence of glucokinase activity, may not be phosphorylated. Addition of glucose led to a small rise in glucose-6-phosphate dehydrogenase activity in the wild type, but constitutive activity was observed in the Δ mutant regardless of the presence of glucose. Microarray analyses showed only small changes in the abundance of global transcripts in following glucose addition, but the transcription levels of several genes, including , but not , were strongly affected by inactivation of . The mechanism(s) whereby Hik31 is involved in glucose sensing and response is discussed.

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2006-03-01
2019-11-22
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